Document Detail


Conservation of mouse alpha A-crystallin promoter activity in chicken lens epithelial cells.
MedLine Citation:
PMID:  1404419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous transfection experiments have shown that 162 base pairs (bp) of the 5' flanking sequence of the chicken alpha A-crystallin gene are required for promoter activity in primary chicken lens epithelial cells (PLE), while only 111 bp of the 5' flanking sequence are needed for activity of the mouse alpha A-crystallin promoter in transfected chicken PLE cells or in a SV40 T-antigen-transformed transfected mouse lens epithelial cell line (alpha TN4-1). The effect of site-directed mutations covering positions -111 to -34 of the mouse alpha A-crystallin promoter fused to the bacterial chloramphenicol acetyltransferase (CAT) gene was compared in transfected chicken PLE cells and mouse alpha TN4-1 cells; selected mutations were also examined in a nontransformed rabbit lens epithelial cell line (N/N1003A). In general, the same mutations reduced promoter activity in the transfected lens cells from all three species, although differences were noted. The mutations severely affected regions -111/-106 and -69/-40 regions in all the transfected cells examined; by contrast, mutations at positions -105/-99 and -87/-70 had a somewhat greater effect in the chicken PLE than the mouse alpha TN4-1 cells, while mutations of the -93/-88 sequence reduced expression in the alpha TN4-1 but not the PLE cells. A partial cDNA with sequence similarity to alpha A-CRYPB1 of the mouse has been isolated from a chicken lens library; mouse alpha A-CRYBP1 is a putative transcription factor which binds to the -66/-55 sequence of the mouse alpha A-crystallin promoter.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
D M Donovan; C M Sax; J F Klement; X Li; A B Chepelinsky; J Piatigorsky
Related Documents :
15934299 - Transfection efficiency comparison of oligonucleotide and plasmid to the hl-60 cell lin...
18455459 - Functional assay of the mutant tissue-nonspecific alkaline phosphatase gene using u2os ...
8822269 - Processing of pro-opiomelanocortin in gh3 cells: inhibition by prohormone convertase 2 ...
16536479 - Cationic nucleoside lipids for gene delivery.
17309779 - The upregulation of expressed proteins in hepg2 cells transfected by the recombinant pl...
19530929 - Relationship between plasmid dna topological forms and in vitro transfection.
23281289 - In vivo quantitative assessment of cell viability of gadolinium or iron-labeled cells u...
20885449 - Effects of sirna-targeting bmp-2 on the abilities of migration and invasion of human li...
21749099 - Top laminated graphene electrode in a semitransparent polymer solar cell by simultaneou...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of molecular evolution     Volume:  35     ISSN:  0022-2844     ISO Abbreviation:  J. Mol. Evol.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-26     Completed Date:  1992-10-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0360051     Medline TA:  J Mol Evol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  337-45     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular and Developmental Biology, NEI, NIH, Bethesda, MD 20892.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Cells, Cultured
Chick Embryo
Cloning, Molecular
Crystallins / chemistry,  genetics*
Gene Expression Regulation / genetics*
Lens, Crystalline / cytology,  metabolism*
Mice
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmids / genetics
Polymerase Chain Reaction
Promoter Regions, Genetic / genetics*
Recombinant Fusion Proteins / chemistry,  genetics*
Transfection
Chemical
Reg. No./Substance:
0/Crystallins; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Six strains of human immunodeficiency virus type 1 isolated in Japan and their molecular phylogeny.
Next Document:  Radionuclide-induced evolution of DNA and the origin of life.