| Conservation of epigenetic regulation, ORC binding and developmental timing of DNA replication origins in the genus Drosophila. | |
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MedLine Citation:
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PMID: 18039868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is much interest in how DNA replication origins are regulated so that the genome is completely duplicated each cell division cycle and in how the division of cells is spatially and temporally integrated with development. In the Drosophila melanogaster ovary, the cell cycle of somatic follicle cells is modified at precise times in oogenesis. Follicle cells first proliferate via a canonical mitotic division cycle and then enter an endocycle, resulting in their polyploidization. They subsequently enter a specialized amplification phase during which only a few, select origins repeatedly initiate DNA replication, resulting in gene copy number increases at several loci important for eggshell synthesis. Here we investigate the importance of these modified cell cycles for oogenesis by determining whether they have been conserved in evolution. We find that their developmental timing has been strictly conserved among Drosophila species that have been separate for approximately 40 million years of evolution and provide evidence that additional gene loci may be amplified in some species. Further, we find that the acetylation of nucleosomes and Orc2 protein binding at active amplification origins is conserved. Conservation of DNA subsequences within amplification origins from the 12 recently sequenced Drosophila species genomes implicates members of a Myb protein complex in recruiting acetylases to the origin. Our findings suggest that conserved developmental mechanisms integrate egg chamber morphogenesis with cell cycle modifications and the epigenetic regulation of origins. |
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Authors:
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B R Calvi; B A Byrnes; A J Kolpakas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Genetics Volume: 177 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-27 Completed Date: 2008-02-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 1291-301 Citation Subset: IM |
Affiliation:
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Department of Biology, Syracuse University, Syracuse, New York 13244, USA. bcalvi@syr.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Animals Base Sequence Cell Cycle Cell Cycle Proteins / metabolism DNA Replication* Drosophila / cytology, genetics*, growth & development, metabolism* Drosophila Proteins / genetics, metabolism* Epigenesis, Genetic* Evolution Female Gene Amplification Molecular Sequence Data Nucleosomes / metabolism Oogenesis / genetics, physiology Origin Recognition Complex / genetics, metabolism* Ovarian Follicle / cytology, metabolism Protein Binding Proto-Oncogene Proteins c-myb / metabolism Sequence Homology, Nucleic Acid Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM061290/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Drosophila Proteins; 0/Myb protein, Drosophila; 0/Nucleosomes; 0/Orc2 protein, Drosophila; 0/Origin Recognition Complex; 0/Proto-Oncogene Proteins c-myb |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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