Document Detail


Conservation of chromosomal arrangement among three strains of the genetically unstable archaeon Halobacterium salinarium.
MedLine Citation:
PMID:  8002597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phenotypic variants of Halobacterium salinarium NRC-1 arise at a frequency of 10(-2). These result from transpositions of halobacterial insertion sequences and rearrangements mediated by halobacterial insertion sequences. We have tested the hypothesis that such mutations are confined to only a portion of the genome by comparing the chromosomal restriction map of H. salinarium NRC-1 and that of the derivative S9, which was made in 1969. The two chromosomes were mapped by using two-dimensional pulsed-field gel electrophoresis and the restriction enzymes AflII, AseI, and DraI. A comparison of the two deduced maps showed a domain of about 210 kbp to be subject to many rearrangements, including an inversion in S9 relative to NRC-1. However, the rest of the chromosome was conserved among NRC-1, S9, and an independent Halobacterium isolate, GRB, previously mapped by St. Jean et al. (A. St. Jean, B. A. Trieselmann, and R. L. Charlebois, Nucleic Acids Res. 22:1476-1483, 1994). This concurs with data from eubacteria suggesting strong selective forces maintaining gene order even in the face of rearrangement events occurring at a high frequency.
Authors:
N R Hackett; Y Bobovnikova; N Heyrovska
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  176     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-25     Completed Date:  1995-01-25     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7711-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Cornell University Medical College, New York, New York 10021.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
Chromosomes, Bacterial*
Electrophoresis, Gel, Pulsed-Field
Gene Rearrangement*
Genetic Variation*
Genome, Bacterial
Halobacterium / classification,  genetics*
Mutagenesis, Insertional
Restriction Mapping
Grant Support
ID/Acronym/Agency:
R29-HG00349/HG/NHGRI NIH HHS
Comments/Corrections

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