| Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis. | |
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MedLine Citation:
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PMID: 415859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes. |
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Authors:
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K Kristiansen; B Hartmann; P Plesner; A Krüger |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 83 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1978 Feb |
Date Detail:
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Created Date: 1978-05-17 Completed Date: 1978-05-17 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 389-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetone Animals Cell Fractionation / methods Molecular Weight Protein Biosynthesis Ribosomal Proteins / analysis Ribosomes / metabolism, ultrastructure* Tetrahymena pyriformis / metabolism, ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Ribosomal Proteins; 67-64-1/Acetone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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