Document Detail


Consequences of p53 gene expression by adenovirus vector on cell cycle arrest and apoptosis in human aortic vascular smooth muscle cells.
MedLine Citation:
PMID:  7487976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p53 shows its tumor suppresser activity by inducing cell cycle arrest and/or apoptosis of tumor cells and these activities are in part mediated by p21 cyclin-dependent kinase inhibitor (also called as WAF1, Cip1 and SDI1). Using human aortic vascular smooth muscle cells, here we demonstrate that adenovirus vector expressing p53-induced p21, cell cycle arrest at G1 and G2/M boundary, and accumulation of cells in G1 subgroup. However, adenovirus vector expressing p21 induced only G1 cell cycle arrest. The adenovirus vector expressing p53 was 200 times more cytotoxic to human aortic vascular smooth muscle cells than adenovirus vector expressing p21. These results suggest that adenovirus expressing p53 induces cytotoxicity in human vascular smooth muscle cells by apoptosis and this cytotoxicity can not be fully accounted by p21 induction.
Authors:
D Katayose; R Wersto; K Cowan; P Seth
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  215     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-11-24     Completed Date:  1995-11-24     Revised Date:  2006-04-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  446-51     Citation Subset:  IM    
Affiliation:
Medicine Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae
Aorta / cytology,  physiology
Apoptosis*
Cell Cycle*
Cell Survival
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis,  metabolism*
DNA / analysis
Enzyme Inhibitors / metabolism
Gene Expression*
Genes, p53*
Genetic Vectors
Humans
Kinetics
Muscle, Smooth, Vascular / cytology*,  physiology*
Transfection
Tumor Suppressor Protein p53 / biosynthesis*
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Enzyme Inhibitors; 0/Tumor Suppressor Protein p53; 9007-49-2/DNA

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