Document Detail

Consequences of nitric oxide synthase inhibition during bovine oocyte maturation on meiosis and embryo development.
MedLine Citation:
PMID:  20137060     Owner:  NLM     Status:  MEDLINE    
The importance of nitric oxide synthase (NOS) in bovine oocyte maturation was investigated. Oocytes were in vitro matured with the NOS inhibitor N(w)-L-nitro-arginine methyl-ester (10(-7), 10(-5) and 10(-3) m L-NAME) and metaphase II (MII) rates and embryo development and quality were assessed. The effect of L-NAME (10(-7) m) during pre-maturation and/or maturation on embryo development and quality was also assessed. L-NAME decreased MII rates (78-82%, p < 0.05) when compared with controls without L-NAME (96%). Cleavage (77-88%, p > 0.05), Day 7 blastocyst rates (34-42%, p > 0.05) and total cell numbers in blastocysts were similar for all groups (146-171 cells, p > 0.05). Day 8 blastocyst TUNEL positive cells (3-4 cells) increased with L-NAME treatment (p < 0.05). For oocytes cultured with L-NAME during pre-maturation and/or maturation, Day 8 blastocyst development (26-34%) and Day 9 hatching rates (15-22%) were similar (p > 0.05) to controls pre-matured and matured without NOS inhibition (33 and 18%, respectively), while total cell numbers (Day 9 hatched blastocysts) increased (264-324 cells, p < 0.05) when compared with the controls (191 cells). TUNEL positive cells increased when NOS was inhibited only during the maturation period (8 cells, p < 0.05) when compared with the other groups (3-4 cells). NO may be involved in meiosis progression to MII and its deficiency during maturation increases apoptosis in embryos produced in vitro. Nitric oxide synthase inhibition during pre-maturation and/or maturation affects embryo quality.
K R L Schwarz; P R L Pires; T H C de Bem; P R Adona; C L V Leal
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  45     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-08     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
Departamento de Ci?ncias B?sicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de S?o Paulo, Pirassununga-SP, Brazil.
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MeSH Terms
Blastocyst / cytology,  drug effects,  physiology
Cell Count
Embryonic Development / drug effects*
Enzyme Inhibitors / pharmacology*
Fertilization in Vitro / veterinary
In Situ Nick-End Labeling
Meiosis / drug effects*
Metaphase / drug effects
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors*
Oocytes / cytology,  physiology*
Reg. No./Substance:
0/Enzyme Inhibitors; 50903-99-6/NG-Nitroarginine Methyl Ester; EC Oxide Synthase

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