Document Detail

Connexin transfection induces invasive properties in HeLa cells.
MedLine Citation:
PMID:  9716458     Owner:  NLM     Status:  MEDLINE    
Gap-junctional coupling of tumor cells facilitates their invasion into normal tissue as was further investigated with connexin-transfected HeLa cells by an in vitro assay. Wild-type HeLa cells are coupling deficient and did not show invasive properties when multicell spheroids were confronted with precultured embryonic chicken heart fragments. After transfection with cDNA of Cx31, Cx40, or Cx43, these cells were homotypically coupled; Cx40- and Cx43-transfected HeLa cells also were heterotypically coupled with embryonic chicken heart cells in monolayer coculture. Transfected clones revealed invasive properties in the chicken heart in vitro assay. However, the pattern of invasion differed between these transfectants: After 4 days Cx43-transfected HeLa cells were found in the central part of heart spheroids; Cx40- and Cx31-transfected HeLa cells, however, did not invade the central core but were detected in the outer part of heart spheroids. We conclude that connexin expression supports invasion of tumor cells into normal tissue, but heterotypic gap-junctional coupling is not required for this process.
S H Graeber; D F Hülser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  243     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-24     Completed Date:  1998-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  142-9     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Biological Institute, University of Stuttgart, Pfaffenwaldring 57, Stuttgart, D-70550, Germany.
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MeSH Terms
Biotin / analogs & derivatives,  analysis
Cell Communication
Cell Division
Cells, Cultured
Chick Embryo
Coculture Techniques
Connexin 43 / genetics
Connexins / genetics,  physiology*
Culture Techniques
Hela Cells / chemistry,  cytology,  metabolism
Myocardium / chemistry,  cytology*,  metabolism
Neoplasm Invasiveness*
Reg. No./Substance:
0/Connexin 43; 0/Connexins; 0/connexin 40; 0/neurobiotin; 136362-16-8/GJB3 protein, human; 58-85-5/Biotin

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