| Connexin mimetic peptides fail to inhibit vascular conducted calcium responses in renal arterioles. | |
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MedLine Citation:
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PMID: 18614765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vascular conducted responses are believed to play a central role in controlling the microcirculatory blood flow. The responses most likely spread through gap junctions in the vascular wall. At present, four different connexins (Cx) have been detected in the renal vasculature, but their role in transmission of conducted vasoconstrictor signals in the preglomerular arterioles is unknown. Connexin mimetic peptides were previously reported to target and inhibit specific connexins. We, therefore, investigated whether conducted vasoconstriction in isolated renal arterioles could be blocked by the use of mimetic peptides directed against one or more connexins. Preglomerular resistance vessels were microdissected from kidneys of Sprague-Dawley rats and loaded with fura 2. The vessels were stimulated locally by applying electrical current through a micropipette, and the conducted calcium response was measured 500 mum from the site of stimulation. Application of connexin mimetic peptides directed against Cx40, 37/43, 45, or a cocktail with equimolar amounts of each, did not inhibit the propagated response, whereas the nonselective gap junction uncoupler carbenoxolone completely abolished the propagated response. However, the connexin mimetic peptides were able to reduce dye coupling between rat aorta endothelial cells shown to express primarily Cx40. In conclusion, we did not observe any attenuating effects on conducted calcium responses in isolated rat interlobular arteries when exposed to connexin mimetic peptides directed against Cx40, 37/43, or 45. Further studies are needed to determine whether conducted vasoconstriction is mediated via previously undescribed pathways. |
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Authors:
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Charlotte Mehlin Sorensen; Max Salomonsson; Thomas Hartig Braunstein; Morten Schak Nielsen; Niels-Henrik Holstein-Rathlou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-09 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 295 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-08 Completed Date: 2008-10-17 Revised Date: 2010-05-26 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R840-7 Citation Subset: IM |
Affiliation:
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Univ. of Copenhagen, Dept. of Biomedical Sciences, The Panum Institute, Bldg. 10.5, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. cmehlin@mfi.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / cytology Arterioles / cytology, physiology Calcium / metabolism* Cell Communication / drug effects, physiology Cells, Cultured Connexin 43 / genetics, pharmacology Connexins / genetics, pharmacology* Electric Stimulation Endothelial Cells / cytology, drug effects*, physiology* Hela Cells Humans Kidney Glomerulus / blood supply* Molecular Mimicry Peptides / pharmacology Rats Rats, Sprague-Dawley Renal Circulation / drug effects, physiology* Transfection Vasoconstriction / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Connexins; 0/Peptides; 0/connexin 37; 0/connexin 40; 0/connexin 45; 7440-70-2/Calcium |
| Comments/Corrections | |
Erratum In:
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Am J Physiol Regul Integr Comp Physiol. 2010 Jan;298(1):R243 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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