Document Detail


Connexin 36 is expressed and associated with zonula occludens-1 protein in PC-12 cells.
MedLine Citation:
PMID:  17579252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Connexin 36 (Cx36) is the predominant connexin isoform expressed in the mammalian neurons of the central nervous system (CNS). PC-12 cells, a neuronal-like cell line, are widely used for neuron functional studies. Many connexins have been shown to interact with zonula occludens-1 protein (ZO-1), a tight junction associated with protein. The present study is intended to investigate whether Cx36 is expressed in PC-12 cells and is associated with ZO-1. Cx36 transcripts were amplified and verified by RT-PCR. 2.9 kb Cx36 mRNA was detected in PC-12 cells through Northern blot hybridization. Western blotting showed a 36-kDa protein band in the homogenates of PC-12 cells. Immunofluorescence labeling revealed that Cx36 was present in cell-cell contacts of PC-12 cells and colocalized with ZO-1. The association of Cx36 and ZO-1 in PC-12 cells was also demonstrated by coimmunoprecipitation. In conclusion, PC-12 cells express Cx36 mRNA and Cx36 proteins that are associated with ZO-1. These results enhanced our understanding of the function of Cx36 in PC-12 cells.
Authors:
S J Lu; H Li; F H Zhou; J J Zhang; L X Wang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  General physiology and biophysics     Volume:  26     ISSN:  0231-5882     ISO Abbreviation:  Gen. Physiol. Biophys.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8400604     Medline TA:  Gen Physiol Biophys     Country:  Slovakia    
Other Details:
Languages:  eng     Pagination:  33-9     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Blotting, Western
Connexins / metabolism*
Fluorescent Antibody Technique, Direct
Immunoprecipitation
In Situ Hybridization
Membrane Proteins / metabolism*
Molecular Weight
Neurons / chemistry,  metabolism*
PC12 Cells / cytology,  metabolism*
Phosphoproteins / metabolism*
RNA, Messenger / metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Connexins; 0/Membrane Proteins; 0/Phosphoproteins; 0/RNA, Messenger; 0/connexin 36; 0/zonula occludens-1 protein

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