| Connexin 36 is expressed and associated with zonula occludens-1 protein in PC-12 cells. | |
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MedLine Citation:
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PMID: 17579252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Connexin 36 (Cx36) is the predominant connexin isoform expressed in the mammalian neurons of the central nervous system (CNS). PC-12 cells, a neuronal-like cell line, are widely used for neuron functional studies. Many connexins have been shown to interact with zonula occludens-1 protein (ZO-1), a tight junction associated with protein. The present study is intended to investigate whether Cx36 is expressed in PC-12 cells and is associated with ZO-1. Cx36 transcripts were amplified and verified by RT-PCR. 2.9 kb Cx36 mRNA was detected in PC-12 cells through Northern blot hybridization. Western blotting showed a 36-kDa protein band in the homogenates of PC-12 cells. Immunofluorescence labeling revealed that Cx36 was present in cell-cell contacts of PC-12 cells and colocalized with ZO-1. The association of Cx36 and ZO-1 in PC-12 cells was also demonstrated by coimmunoprecipitation. In conclusion, PC-12 cells express Cx36 mRNA and Cx36 proteins that are associated with ZO-1. These results enhanced our understanding of the function of Cx36 in PC-12 cells. |
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Authors:
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S J Lu; H Li; F H Zhou; J J Zhang; L X Wang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: General physiology and biophysics Volume: 26 ISSN: 0231-5882 ISO Abbreviation: Gen. Physiol. Biophys. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-06-20 Completed Date: 2007-08-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8400604 Medline TA: Gen Physiol Biophys Country: Slovakia |
Other Details:
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Languages: eng Pagination: 33-9 Citation Subset: IM |
Affiliation:
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Department of Histology and Embryology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Blotting, Western Connexins / metabolism* Fluorescent Antibody Technique, Direct Immunoprecipitation In Situ Hybridization Membrane Proteins / metabolism* Molecular Weight Neurons / chemistry, metabolism* PC12 Cells / cytology, metabolism* Phosphoproteins / metabolism* RNA, Messenger / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Connexins; 0/Membrane Proteins; 0/Phosphoproteins; 0/RNA, Messenger; 0/connexin 36; 0/zonula occludens-1 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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