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Connexin 31 (GJB3) Deficiency in Mouse Trophoblast Stem Cells Alters Giant Cell Differentiation and Leads to Loss of Oxygen Sensing.
MedLine Citation:
PMID:  22623621     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A nonphysiological placental oxidative environment has been implicated in many complications during human pregnancy. Oxygen tension can influence a broad spectrum of molecular changes leading to alterations in trophoblast cell lineage development. In this study we report that mouse wild type trophoblast stem cells (TSCs) react to low oxygen (3%) with an enhanced differentiation into the giant cell pathway indicated by a downregulation of the early stem cell markers Eomes and Cdx2 as well as by a significant upregulation of Tfap2c and the differentiation markers Tpbpa and Prl3d1. Here we demonstrated that connexin 31/GJB3 deficient TSCs failed to stabilize HIF-1A under low oxygen resulting in non-responsiveness of different marker genes such as Cdx2 and Eomes, Tfap2c and Tpbpa. Moreover, connexin 31 deficient TSCs revealed a shift in giant cell differentiation from Prl3d1 expressing parietal giant cells to Ctsq, Prl3b1 and Prl2c2 positive giant cells probably sinusoidal and canal lining trophoblast giant cells. Thus loss of CX31 led to different giant cell subtypes which bypass the progenitor regulators Tfap2c and Tpbpa under low oxygen.
Authors:
Yvonne Koch; Betina van Fürden; Stephanie Kaiser; Diana Klein; Mark Kibschull; Hubert Schorle; Alexander Carpinteiro; Alexandra Gellhaus; Elke Winterhager
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-23
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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