Document Detail

Connective tissue changes in a mouse model of vein graft disease.
MedLine Citation:
PMID:  18431349     Owner:  NLM     Status:  MEDLINE    
AIM: The extracellular matrix plays an important physiological role in the architecture of the vascular wall. In arterialized vein grafts severe early changes, such as thrombosis and neointimal hyperplasia occur. Paclitaxel is in clinical use as antiproliferative coating of coronary stents. We aimed to investigate the early connective tissue changes in arterialized vein grafts and the influence of perivascular paclitaxel treatment in an in vivo model. METHODS: C57 black mice underwent interposition of the vena cava into the carotid artery. Neointimal hyperplasia, thrombosis, acid mucopolysaccharides (Alcian), collagen fibers (trichrome Masson), elastic fibers, and apoptosis rate (TUNEL) were quantified in paclitaxel treated veins and controls. RESULTS: In both, controls and paclitaxel treated vein grafts acid mucopolysaccharides and elastic fibers were found predominantly in the neointima, whereas collagen fibers were found mainly in the media and adventitia. At 4 weeks postoperatively the neointimal thickness in controls was 52 (13-130) microm, whereas in 0.6 mg/mL l paclitaxel treated veins it was 103 (43-318) microm (P=0.094). At 8 weeks postoperatively paclitaxel treated veins showed a significantly increased neointimal thickness of 136 (87-199) microm compared with 79 (62-146) microm in controls (P=0.032). There was no difference in apoptosis rate between the two groups (P=NS). Even with the lowest concentration of 0.008 mg/mL paclitaxel veins showed a neointimal thickness of 67 (46-205) microm at 4 weeks postoperatively (P=NS vs controls). CONCLUSION: Early vein graft disease is characterised by an accumulation of acid mucopolysaccharides and elastic fibers in the thickened neointima. Paclitaxel treatment increases the neointimal hyperplasia in mouse vein grafts in vivo.
T Schachner; S Heiss; T Mayr; C Steger; D Zipponi; P Reisinger; N Bonaros; G Laufer; J Bonatti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cardiovascular surgery     Volume:  49     ISSN:  0021-9509     ISO Abbreviation:  J Cardiovasc Surg (Torino)     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-23     Completed Date:  2008-07-18     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0066127     Medline TA:  J Cardiovasc Surg (Torino)     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
Department of Cardiac Surgery, Innsbruck Medical University, Innsbruck, Austria.
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MeSH Terms
Apoptosis / drug effects
Carotid Arteries / surgery
Collagen / metabolism
Connective Tissue / metabolism,  pathology*
Glycosaminoglycans / metabolism
Graft Occlusion, Vascular / pathology,  physiopathology
In Situ Nick-End Labeling
Mice, Inbred C57BL
Paclitaxel / pharmacology
Thrombosis / chemically induced
Tunica Intima / drug effects,  pathology
Vena Cava, Inferior / drug effects,  pathology,  transplantation*
Reg. No./Substance:
0/Glycosaminoglycans; 33069-62-4/Paclitaxel; 9007-34-5/Collagen

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