Document Detail

Connective tissue cells, cell proliferation and synthesis of extracellular matrix-a review.
MedLine Citation:
PMID:  239419     Owner:  NLM     Status:  MEDLINE    
The ubiquitous connective tissues contain a wide range of cells including fibroblasts, osteoblasts and chondroblasts. Recently it has been demonstrated that another principal cell of the connective tissue is the smooth muscle cell in several organ systems. These have been shown to be responsible for the synthesis of the connective tissue matrix components of the uterine myometrium and of the arterial system, including collagen, both elastic fibre proteins and glycosaminoglycan. Microtubule inhibitors such as colchicine and vinblastine, and iron chelators such as alpha,alpha -dipyridyl have been used to study their morphologic and chemical effects on collagen synthesis and secretion. Colchicine produces an increase in large Golgi-associated vacuoles, which sometimes contain material reminiscent of aggregates of collagen macromolecules. Vinblastine produces alterations in the endoplasmic reticulum cisternae similar to alterations seen in ascorbic acid deficiency, and alpha,alpha-dipyridyl increases the frequency of regions in cells, interpretable as potential sites of communication of rough endoplasmic reticulum cisternae with the cell surface. Ferritin conjugated anti-procallagen sera were used to localize procollagen in cells and demonstrated procollagen not only in the cisternae of rough endoplasmic reticulum but in all of the elements of the Golgi complex as well. The studies reported in this review have shown that in cell culture arterial smooth muscle will produce not only the microfibrillar protein of the elastic fibre but soluble and/or insoluble elastin as well. Recent studies on serum factors responsible for the proliferation of connective tissue cells have demonstrated that at least one of the principal factors responsible for fibroblast and/or smooth muscle cell proliferation in culture is derived from thrombocytes. Medium containing serum derived from cell-free plasma lacks most of this proliferative effect which can be reinstated when platelets are present during recalcification to form serum. This effect is due to the platelet release reaction as shown by combining supernatant factors derived from platelets exposed to purified thrombin to cell-free, plasma derived serum. Studies with macrophages have also suggested that phagocytic macrophages release factor(s) into a cell culture medium that may also participate in stimulating fibroblasts to proliferate in vitro. The means by which these factors stimulate fibroblast proliferation and connective tissue synthesis remains to be elucidated.
R Ross
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Philosophical transactions of the Royal Society of London. Series B, Biological sciences     Volume:  271     ISSN:  0962-8436     ISO Abbreviation:  Philos. Trans. R. Soc. Lond., B, Biol. Sci.     Publication Date:  1975 Jul 
Date Detail:
Created Date:  1975-11-08     Completed Date:  1975-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503623     Medline TA:  Philos Trans R Soc Lond B Biol Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  247-59     Citation Subset:  IM    
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MeSH Terms
Cell Division
Chick Embryo
Colchicine / pharmacology
Collagen / analysis,  biosynthesis*,  secretion
Connective Tissue / analysis,  drug effects,  metabolism*
Connective Tissue Cells*
Elastin / analysis,  biosynthesis,  secretion
Extracellular Space / metabolism*
Fibroblasts / metabolism,  physiology*,  secretion
Glycosaminoglycans / analysis
Iron Chelating Agents / pharmacology
Microscopy, Electron
Osteoblasts / secretion,  ultrastructure
Vinblastine / pharmacology
Reg. No./Substance:
0/Glycosaminoglycans; 0/Iron Chelating Agents; 64-86-8/Colchicine; 865-21-4/Vinblastine; 9007-34-5/Collagen; 9007-58-3/Elastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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