Document Detail


Connective tissue activation. XXX: Isoelectric point microheterogeneity of CTAP-III, a human platelet-derived growth factor.
MedLine Citation:
PMID:  3081909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Connective tissue activating peptide (CTAP-III) is one of the growth factors found in the alpha granules of human platelets. This small cationic platelet protein shows several apparent isoelectric point variants after the preparative isoelectric focusing step in large scale preparations from multiple donors. Analytical isoelectricfocusing techniques under denaturing conditions, coupled with Western blotting and immunodetection, now show that CTAP-III prepared rapidly from single donors also has multiple isoelectric point variants, suggesting that this finding is more likely related to post-translational modification than preparative artifact or genetic polymorphism.
Authors:
M S Green; P A Hossler; C W Castor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  181     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-04-18     Completed Date:  1986-04-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Platelets / analysis
Electrophoresis, Polyacrylamide Gel
Growth Substances / analysis*
Humans
Immunodiffusion
Isoelectric Point
Peptides / analysis*
Platelet-Derived Growth Factor / analysis*
Grant Support
ID/Acronym/Agency:
AM-10728/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Growth Substances; 0/Peptides; 0/Platelet-Derived Growth Factor; 69344-77-0/connective tissue-activating peptide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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