Document Detail

Conjugated polythiophene probes target lysosome-related acidic vacuoles in cultured primary cells.
MedLine Citation:
PMID:  17553666     Owner:  NLM     Status:  MEDLINE    
Conformation-sensitive optical probes for studying biological processes and structures are of great interest. The present work shows for the first time that conjugated polyelectrolyte (CPE) probes can be used for specific targeting of chromatin, nuclear and cytoplasmatic vesicles, and cytoskeletal components in a complex system of cultured cells. One of the probes could also be used for vital staining of live cells. When bound to different entities, the polythiophene derivative probes emitted light with different colors due to the unique spectral properties of these conformation sensitive probes. The physical pre-requisites for binding could also be exploited for characterization of the target. Unexpectedly, lysosome-related acidic vacuoles were targeted in cultured primary cells by both anionic, cationic, and zwitter-ionic polythiophene derivatives. Pre-treatment with Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, caused redistribution of the staining. The targeting of lysosome-related acidic vesicles could not be demonstrated in transformed cells (melanoma, neuroblastoma, and prostate cancer cell lines), indicating a difference in the localization, structure, accessibility, or quantity of the target in cultured normal cells as compared with the malignant cell lines. The chemical nature of the conjugated polyelectrolyte complex in the cytoplasmatic vacuoles remains elusive.
Per Björk; K Peter R Nilsson; Liselotte Lenner; Bertil Kågedal; Birgitta Persson; Olle Inganäs; Jon Jonasson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-27
Journal Detail:
Title:  Molecular and cellular probes     Volume:  21     ISSN:  0890-8508     ISO Abbreviation:  Mol. Cell. Probes     Publication Date:    2007 Oct-Dec
Date Detail:
Created Date:  2007-08-21     Completed Date:  2007-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709751     Medline TA:  Mol Cell Probes     Country:  England    
Other Details:
Languages:  eng     Pagination:  329-37     Citation Subset:  IM    
Biomolecular and Organic Electronics, IFM, Linköping University, SE-581 83 Linköping, Sweden.
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MeSH Terms
Cell Survival / drug effects
Cell Transformation, Neoplastic / drug effects
Cells, Cultured
Lysosomes / drug effects,  metabolism*
Macrolides / pharmacology
Mice, Inbred C57BL
Microscopy, Fluorescence
Molecular Probes / chemistry,  metabolism*
Nucleic Acids / metabolism
Polymers / chemistry,  metabolism*
Thiophenes / chemistry,  metabolism*
Tissue Fixation
Vacuoles / drug effects,  metabolism*
Reg. No./Substance:
0/Anions; 0/Cations; 0/Macrolides; 0/Molecular Probes; 0/Nucleic Acids; 0/Polymers; 0/Thiophenes; 25233-34-5/polythiophene; 88899-55-2/bafilomycin A1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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