| Conjugated polyene fatty acids as fluorescent probes: biosynthetic incorporation of parinaric acid by Escherichia coli and studies of phase transitions. | |
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MedLine Citation:
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PMID: 321009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of the fluorescent fatty acid, parinaric acid (9, 11, 13, 15-octadecatetraenoic acid) (PnA), was studied in cells of an unsaturated fatty acid auxotroph of Escherichia coli. Growth conditions were found that permitted biosynthetic incorporation of PnA (up to 3%) into membrane phospholipids during growth on oleic or elaidic acid. Fluorescence measurements of incorporated PnA revealed phase transitions in cells, membranes, and phospholipids at temperatures that reflected the fatty acid composition of the sample. Transitions had a well-defined onset from high temperature, while the lower and end point was less well defined. cis- and trans-PnA (cis, trnas, trans, cis, and all trans, respectively) gave comparable results. Similar phase transitions were detected with PnA, which was not biosynthetically incorporated. Fluorescence of tryptophan was measured in E. coli membranes as a function of concentration of PnA. Significant quenching of tryptophan fluorescence by PnA was observed. |
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Authors:
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E S Tecoma; L A Sklar; R D Simoni; B S Hudson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 16 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1977 Mar |
Date Detail:
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Created Date: 1977-05-12 Completed Date: 1977-05-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 829-35 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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metabolism Escherichia coli / metabolism* Fatty Acids, Unsaturated / metabolism* Fluorescent Dyes Kinetics Membrane Lipids / metabolism Polyenes / metabolism* Spectrometry, Fluorescence Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Fluorescent Dyes; 0/Membrane Lipids; 0/Polyenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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