Document Detail

Conjugated polyene fatty acids as membrane probes: preliminary characterization.
MedLine Citation:
PMID:  1057769     Owner:  NLM     Status:  MEDLINE    
The use of fluorescent conjugated polyenoic fatty acids as probes of membrane structure is introduced. alpha- and beta-parinaric acid (cis, trans, trans, cis-, and all trans-9,11,13,15-octadecatetraenoic acid) and synthetic lecithins containing an alpha-parinaric acid chain in position 2 are prepared and their absorption and fluorescence properties are determined. Phase transitions are detected as fluorescence changes at characteristic temperatures when either the free fatty acid probes or the labeled phospholipid probe are included in sonicated aqueous dispersions of L-alpha-dimyristoyl lecithin, L-alpha dipalmitoyl lecithin, or L-alpha-distearoyl lecithin. The phase transitions are detected at about 23 degrees C (dimyristoyl), 44 degrees C (dipalmitoyl), and 53 degrees C (distearoyl lecithin). Binding of alpha-parinaric acid to bovine serum albumin is measured by shifts in the absorption spectrum and enhanced quantum yield of the fatty acid upon binding and by energy transfer between 2 tryptophyl residues in bovine serum albumin and alpha-parinaric acid. Approximately six binding sites are detected. Other applications of these probe molecules, including phase transitions of phospholipid/cholesterol dispersions, induced circular dichroism of parinaric acid bound to albumin, and biosynthetic incorporation of parinaric acid into biological membranes, are discussed.
L A Sklar; B S Hudson; R D Simoni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  72     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-11-25     Completed Date:  1975-11-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1649-53     Citation Subset:  IM    
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MeSH Terms
Fatty Acids, Unsaturated*
Membranes, Artificial*
Models, Biological
Models, Molecular
Molecular Conformation
Myristic Acids
Palmitic Acids
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Stearic Acids
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Membranes, Artificial; 0/Myristic Acids; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Polyenes; 0/Stearic Acids

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