Document Detail

Conjugated and non-conjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: cholesterol acyltransferase activity.
MedLine Citation:
PMID:  18078940     Owner:  NLM     Status:  MEDLINE    
We investigated the relative hypocholesterolemic activity of linoleic acid (LA), conjugated linoleic acid (CLA), alpha-linolenic acid (LN) and conjugated linolenic acid (CLN) in hamsters. Five groups of hamsters (n=10 each) were fed either the control diet or one of the four fatty acids-supplemented diets for 6 weeks. Results demonstrated that the four octadecaenoic acids decreased plasma cholesterol differently, with CLA being the most effective. Western blotting and RT-PCR analysis demonstrated that the four octadecaenoic acids had no effect on sterol regulatory element binding protein-2 (SREBP-2), liver X receptor (LXR), 3-hydroxy-3-methylglutary-CoA reductase (HMGR), LDL receptor (LDLR), and cholesterol-7alpha-hydroxylase (CYP7A1). However, the four octadecaenoic acids increased the excretion of fecal neutral sterols with CLA being most effective followed by LN, LA and CLN, suggesting they all differentially affect cholesterol absorption. Dietary CLA was associated with the least intestinal acyl coenzyme A: cholesterol acyltransferase (ACAT) activity followed by LN, LA and CLN in a decreasing trend. Since esterification of cholesterol is catalyzed by intestinal ACAT, and is a rate-limiting step in cholesterol absorption, it was concluded that the varying effects of CLA, LN, LA and CLN on blood cholesterol were mediated, at least in part, by their inhibition on intestinal ACAT activity.
Cheuk Kai Lam; Jingnan Chen; Ying Cao; Lin Yang; Yin Mei Wong; Sai Ying Venus Yeung; Xiaoqiang Yao; Yu Huang; Zhen-Yu Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-21
Journal Detail:
Title:  Atherosclerosis     Volume:  198     ISSN:  1879-1484     ISO Abbreviation:  Atherosclerosis     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  85-93     Citation Subset:  IM    
Food & Nutritional Sciences Programme of Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
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MeSH Terms
Acyl Coenzyme A / metabolism*
Body Weight
Cholesterol 7-alpha-Hydroxylase / genetics,  metabolism
Cholesterol, HDL / blood
DNA-Binding Proteins / genetics,  metabolism
Fatty Acids, Unsaturated / pharmacology*
Hydroxymethylglutaryl CoA Reductases / genetics,  metabolism
Intestines / drug effects,  enzymology*
Linoleic Acids, Conjugated / pharmacology*
Liver / metabolism
Organ Size
Orphan Nuclear Receptors
RNA, Messenger / metabolism
Receptors, Cytoplasmic and Nuclear / genetics,  metabolism
Receptors, LDL / genetics,  metabolism
Sterol O-Acyltransferase / metabolism*
Sterol Regulatory Element Binding Protein 2 / genetics,  metabolism
Sterols / metabolism
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Cholesterol, HDL; 0/DNA-Binding Proteins; 0/Fatty Acids, Unsaturated; 0/Linoleic Acids, Conjugated; 0/Orphan Nuclear Receptors; 0/RNA, Messenger; 0/Receptors, Cytoplasmic and Nuclear; 0/Receptors, LDL; 0/Sterol Regulatory Element Binding Protein 2; 0/Sterols; 0/liver X receptor; 26764-25-0/octadecadienoic acid; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases; EC 7-alpha-Hydroxylase; EC O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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