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Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes.
MedLine Citation:
PMID:  22555954     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Conjugated linoleic acid (CLA) is a polyunsaturated fatty acid, which has been recently proven to be effective in reducing body fat mass, but brings as a side effect, the liver enlargement due to an increased lipid content. The in vivo lipogenic activity has been suggested to be due to the reduction in fat mass and to the consequent metabolism of blood glucose to fatty acid in the liver rather than in the adipose tissue. We investigated the ability of CLA to directly induce steatosis by modulating the expression pattern of hepatic proteins involved in lipid metabolism. To avoid interferences derived from CLA metabolism by other tissues, we used the in vitro model of freshly isolated rat hepatocytes incubated in the presence of different CLA isomers. The direct effect of CLA on lipid accumulation in hepatocytes was demonstrated by the altered expression pattern of several proteins involved in lipid metabolism, as assessed by two-dimensional gel electrophoresis and confirmed by Western blotting analysis. The CLA isomer c9,t11 was most effective in modulating the protein expression profile.
Authors:
E Rossi; L Della Casa; S Piana; A Iannone
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Publication Detail:
Type:  Journal Article     Date:  2012-05-05
Journal Detail:
Title:  Genes & nutrition     Volume:  7     ISSN:  1555-8932     ISO Abbreviation:  Genes Nutr     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-21     Completed Date:  2012-10-02     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  101280108     Medline TA:  Genes Nutr     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  511-27     Citation Subset:  -    
Affiliation:
"ProteoWork Lab", Dipartimento di Scienze Biomediche, Università di Modena e Reggio Emilia, via Campi 287, 41125, Modena, Italy.
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