| Conjugated linoleic acid inhibits Caco-2 cell growth via ERK-MAPK signaling pathway. | |
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MedLine Citation:
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PMID: 16963252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Conjugated linoleic acid (CLA) is a naturally occurring compound found in dairy and beef products. In recent years, it has received considerable attention because several studies showed a lower incidence of certain cancers in animals fed CLA-supplemented diets. In vitro studies further showed growth inhibitory activity on tumor cell proliferation, the CLA being effective above all against colon cancer cells. The aim of the present work was to investigate the growth inhibitory effect of CLA on Caco-2 cell line. Under our experimental conditions, CLA repressed Caco-2 cell proliferation, and the growth-inhibitory action increased by repeating treatments. However, in Caco-2 cells, CLA was unable to induce apoptosis, as revealed by cell-cycle analysis and Western blot studies. To determine the mechanism by which CLA inhibits cell growth, we studied its effect on extracellular-regulated kinase signaling. Conjugated linoleic acid reduced expression levels of Raf-1 and phosphorylation of ERK1/2, which was accompanied by a decrease in the expression of the downstream transcription factor c-myc. Our data suggest that CLA is dependent, at least in part, on the ERK kinase pathway for its ability to inhibit the growth of Caco-2 cancer cells. |
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Authors:
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Claudia Bocca; Francesca Bozzo; Ludovica Gabriel; Antonella Miglietta |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-08 |
Journal Detail:
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Title: The Journal of nutritional biochemistry Volume: 18 ISSN: 0955-2863 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-23 Completed Date: 2007-07-20 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 332-40 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine and Oncology, University of Torino, 10125, Torino, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology Caco-2 Cells / drug effects* Caspase 3 / drug effects, metabolism Cell Cycle / drug effects Cell Proliferation / drug effects Dose-Response Relationship, Drug Extracellular Signal-Regulated MAP Kinases / drug effects*, metabolism Humans L-Lactate Dehydrogenase / drug effects, metabolism Linoleic Acids, Conjugated / pharmacology* MAP Kinase Signaling System / drug effects* Proto-Oncogene Proteins c-raf / drug effects, metabolism Signal Transduction bcl-2 Homologous Antagonist-Killer Protein / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/BAK1 protein, human; 0/Linoleic Acids, Conjugated; 0/bcl-2 Homologous Antagonist-Killer Protein; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.11.1/Proto-Oncogene Proteins c-raf; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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