Document Detail

Conjugated linoleic acid in ewe milk fat.
MedLine Citation:
PMID:  16223456     Owner:  NLM     Status:  MEDLINE    
Ewe milk fat from five different herds was studied to determine the content of conjugated linoleic acid (CLA) isomers. Research was carried out by combining gas chromatography-mass spectrometry (GC-MS) of fatty acid methyl esters (FAME) and 4,4-dimethyloxazolyne derivatives (DMOX) with silver ion-high performance liquid chromatography (Ag+-HPLC). Reconstructed mass spectral profiles of CLA characteristic ions from DMOX were used to identify positional isomers and Ag+-HPLC to quantify them. Total CLA content varied from 0.57 to 0.97 g/100 g of total fatty acids. FAME and DMOX were separated into a complex mixture of minor isomers and major rumenic acid (9-cis 11-trans C18:2) by GC-MS using a 100-m polar capillary column. Rumenic acid would represent more than 75% of total CLA. 11-trans 13-trans, 11-13 cis/trans plus trans/cis and 7-9 cis/trans plus trans/cis were the main CLA isomers after rumenic acid. Minor amounts of 8-10 and 10-12 C18:2 isomers were also found. Although most of the isomers were present in each herd's milk, differences in content were observed for some CLA species.
Pilar Luna; Javier Fontecha; Manuela Juárez; Miguel Angel de la Fuente
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of dairy research     Volume:  72     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-14     Completed Date:  2006-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  415-24     Citation Subset:  IM    
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain.
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MeSH Terms
Chromatography, High Pressure Liquid / methods,  veterinary*
Gas Chromatography-Mass Spectrometry / methods,  veterinary*
Linoleic Acids, Conjugated / analysis*,  chemistry
Lipids / analysis,  chemistry
Milk / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Linoleic Acids, Conjugated; 0/Lipids; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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