| Conjugated linoleic acid in ewe milk fat. | |
| | |
MedLine Citation:
|
PMID: 16223456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ewe milk fat from five different herds was studied to determine the content of conjugated linoleic acid (CLA) isomers. Research was carried out by combining gas chromatography-mass spectrometry (GC-MS) of fatty acid methyl esters (FAME) and 4,4-dimethyloxazolyne derivatives (DMOX) with silver ion-high performance liquid chromatography (Ag+-HPLC). Reconstructed mass spectral profiles of CLA characteristic ions from DMOX were used to identify positional isomers and Ag+-HPLC to quantify them. Total CLA content varied from 0.57 to 0.97 g/100 g of total fatty acids. FAME and DMOX were separated into a complex mixture of minor isomers and major rumenic acid (9-cis 11-trans C18:2) by GC-MS using a 100-m polar capillary column. Rumenic acid would represent more than 75% of total CLA. 11-trans 13-trans, 11-13 cis/trans plus trans/cis and 7-9 cis/trans plus trans/cis were the main CLA isomers after rumenic acid. Minor amounts of 8-10 and 10-12 C18:2 isomers were also found. Although most of the isomers were present in each herd's milk, differences in content were observed for some CLA species. |
| | |
Authors:
|
Pilar Luna; Javier Fontecha; Manuela Juárez; Miguel Angel de la Fuente |
Related Documents
:
|
18038986 - Changes in the fatty acid profile of the subcutaneous fat of swine throughout fattening... 23503546 - Effect of nicotine on the pharmacokinetics of levodopa. 19368396 - Oxidative stability of conjugated linolenic acids. 16544876 - Development of an alternative eluent system for ag+-hplc analysis of conjugated linolei... 17969136 - Linear copolymeric poly(thia-alkanedioates) by lipase-catalyzed esterification and tran... 18031966 - Evaluation of five methods for derivatization and gc determination of a mixture of very... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of dairy research Volume: 72 ISSN: 0022-0299 ISO Abbreviation: J. Dairy Res. Publication Date: 2005 Nov |
Date Detail:
|
Created Date: 2005-10-14 Completed Date: 2006-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: England |
Other Details:
|
Languages: eng Pagination: 415-24 Citation Subset: IM |
Affiliation:
|
Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid / methods, veterinary* Female Gas Chromatography-Mass Spectrometry / methods, veterinary* Isomerism Linoleic Acids, Conjugated / analysis*, chemistry Lipids / analysis, chemistry Milk / chemistry* Reproducibility of Results Sensitivity and Specificity Sheep Silver |
| Chemical | |
Reg. No./Substance:
|
0/Linoleic Acids, Conjugated; 0/Lipids; 7440-22-4/Silver |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Visual scoring of clots in foremilk.
Next Document: Economic impact of reduced milk production associated with Johne's disease on dairy operations in th...