Document Detail

Conjugated linoleic acid depresses the delta9 desaturase index and stearoyl coenzyme A desaturase enzyme activity in porcine subcutaneous adipose tissue.
MedLine Citation:
PMID:  12211379     Owner:  NLM     Status:  MEDLINE    
Conjugated linoleic acid (CLA) has been shown to have an effect on subcutaneous fatty acid composition and has been reported to decrease stearoyl coenzyme A desaturase (SCD) activity by decreasing mRNA expression and(or) catalytic activity in rodents and rodent cell lines. This investigation was designed to study the effects of CLA, corn oil, or beef tallow supplementation on s.c. adipose tissue fatty acid composition, adiposity, SCD enzyme activity, and the delta9 desaturase index in piglets. Eighteen crossbred barrows 16 to 18 d of age were adapted to diet for 1 wk and then assigned randomly to one of three treatments: 1.5% added CLA, 1.5% added corn oil, or 1.5% added beef tallow. Barrows were penned individually and fed the supplemental oils for 35 d (to 25.6 +/- 0.6 kg BW). Subcutaneous adipose tissue samples were obtained after slaughter. Fatty acid composition of the s.c. adipose tissue differed for each fatty acid measured due to diet with the exception of 18:3. The concentrations of CLA trans-10, cis-12 and cis-9, trans-11 were elevated from nondetectable to 1.62 and 2.52 g/100 g lipid, respectively (P < 0.001 for both isomers). Conjugated linoleic acid decreased the delta9 desaturase index (P < 0.01) and SCD enzyme activity, expressed as nanomoles of palmitate converted to palmitoleate/(7 min x g of tissue) (P = 0.075) and nanomoles of palmitate converted to palmitoleate/(7 min 105 cells) (P= 0.056). Tallow-fed pigs had a greater proportion of large adipocytes (> 700 pL) and the greatest SCD activity. These data provide the first direct evidence that dietary CLA depresses SCD enzyme activity in porcine adipose tissue, which may in part be responsible for the depression of adiposity by CLA observed by others in market weight pigs.
S B Smith; T S Hively; G M Cortese; J J Han; K Y Chung; P Casteñada; C D Gilbert; V L Adams; H J Mersmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of animal science     Volume:  80     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-09-04     Completed Date:  2002-11-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2110-5     Citation Subset:  IM    
Department of Animal Science, Texas Agricultural Experiment Station, Texas A&M University, College Station 77843, USA.
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MeSH Terms
Adipose Tissue / chemistry*,  enzymology*
Animal Feed
Corn Oil
Dietary Fats / administration & dosage,  pharmacology
Fatty Acids / analysis*
Linoleic Acid / pharmacology*
Random Allocation
Stearoyl-CoA Desaturase / metabolism*
Swine / metabolism*
Reg. No./Substance:
0/Dietary Fats; 0/Fats; 0/Fatty Acids; 2197-37-7/Linoleic Acid; 61789-97-7/tallow; 8001-30-7/Corn Oil; EC Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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