| Conjugated linoleic acid, cis-9,trans-11, is a substrate for pulmonary 15-lipoxygenase-1 in rat. | |
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MedLine Citation:
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PMID: 16131140 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to determine whether two of the major conjugated linoleic acid (CLA) isomers, cis-9,trans-11 (c9,t11) and trans-10,cis-12 (t10,c12), are possible substrates for pulmonary 15-lipoxygenase-1 (15-LOX-1) and, therefore, they are also involved in the production of 13(S)-hydroxyoctadecadienoic acid [13(S)-HODE] in biological systems. 13(S)-HODE, a major bioactive metabolite of linoleic acid, is an important intracellular signal agent and is involved in cell proliferation and differentiation in various biological systems. Nordihydroguaiaretic acid (NDGA), a known LOX inhibitor, was used as a control for measuring 15-LOX-1 enzyme activity. It was found that c9,t11-CLA was 25% as active as linoleic acid as a substrate for 15-LOX-1; however, t10,c12-CLA was not a substrate for 15-LOX-1 as measured by 13(S)-HODE production. The authenticity of the production of 13(S)-HODE from c9,t11 as a substrate was established by isolation and cochromatography with pure standard on HPLC using non-radioactive and [14C]-c9,t11-CLA. |
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Authors:
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Hyejung Cho; Daniel D Gallaher; A Saari Csallany |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 53 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-31 Completed Date: 2005-10-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7262-6 Citation Subset: IM |
Affiliation:
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Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St. Paul, Minnesota 55108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonate 15-Lipoxygenase / antagonists & inhibitors, metabolism* Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Linoleic Acids / metabolism Linoleic Acids, Conjugated / metabolism* Lung / enzymology* Male Nordihydroguaiaretic Acid / pharmacology Rats Rats, Wistar Stereoisomerism Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 500-38-9/Nordihydroguaiaretic Acid; 5204-88-6/13-hydroxy-9,11-octadecadienoic acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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