Document Detail


Conjugated linoleic acid, cis-9,trans-11, is a substrate for pulmonary 15-lipoxygenase-1 in rat.
MedLine Citation:
PMID:  16131140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to determine whether two of the major conjugated linoleic acid (CLA) isomers, cis-9,trans-11 (c9,t11) and trans-10,cis-12 (t10,c12), are possible substrates for pulmonary 15-lipoxygenase-1 (15-LOX-1) and, therefore, they are also involved in the production of 13(S)-hydroxyoctadecadienoic acid [13(S)-HODE] in biological systems. 13(S)-HODE, a major bioactive metabolite of linoleic acid, is an important intracellular signal agent and is involved in cell proliferation and differentiation in various biological systems. Nordihydroguaiaretic acid (NDGA), a known LOX inhibitor, was used as a control for measuring 15-LOX-1 enzyme activity. It was found that c9,t11-CLA was 25% as active as linoleic acid as a substrate for 15-LOX-1; however, t10,c12-CLA was not a substrate for 15-LOX-1 as measured by 13(S)-HODE production. The authenticity of the production of 13(S)-HODE from c9,t11 as a substrate was established by isolation and cochromatography with pure standard on HPLC using non-radioactive and [14C]-c9,t11-CLA.
Authors:
Hyejung Cho; Daniel D Gallaher; A Saari Csallany
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-31     Completed Date:  2005-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7262-6     Citation Subset:  IM    
Affiliation:
Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St. Paul, Minnesota 55108, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate 15-Lipoxygenase / antagonists & inhibitors,  metabolism*
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Linoleic Acids / metabolism
Linoleic Acids, Conjugated / metabolism*
Lung / enzymology*
Male
Nordihydroguaiaretic Acid / pharmacology
Rats
Rats, Wistar
Stereoisomerism
Substrate Specificity
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 500-38-9/Nordihydroguaiaretic Acid; 5204-88-6/13-hydroxy-9,11-octadecadienoic acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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