Document Detail


Conjugated linoleic acid is a preferential substrate for fatty acid nitration.
MedLine Citation:
PMID:  23144452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oxidation and nitration of unsaturated fatty acids by oxides of nitrogen yield electrophilic derivatives that can modulate protein function via post-translational protein modifications. The biological mechanisms accounting for fatty acid nitration and the specific structural characteristics of products remain to be defined. Herein, conjugated linoleic acid (CLA) is identified as the primary endogenous substrate for fatty acid nitration in vitro and in vivo, yielding up to 10(5) greater extent of nitration products as compared with bis-allylic linoleic acid. Multiple enzymatic and cellular mechanisms account for CLA nitration, including reactions catalyzed by mitochondria, activated macrophages, and gastric acidification. Nitroalkene derivatives of CLA and their metabolites are detected in the plasma of healthy humans and are increased in tissues undergoing episodes of ischemia reperfusion. Dietary CLA and nitrite supplementation in rodents elevates NO(2)-CLA levels in plasma, urine, and tissues, which in turn induces heme oxygenase-1 (HO-1) expression in the colonic epithelium. These results affirm that metabolic and inflammatory reactions yield electrophilic products that can modulate adaptive cell signaling mechanisms.
Authors:
Gustavo Bonacci; Paul R S Baker; Sonia R Salvatore; Darla Shores; Nicholas K H Khoo; Jeffrey R Koenitzer; Dario A Vitturi; Steven R Woodcock; Franca Golin-Bisello; Marsha P Cole; Simon Watkins; Claudette St Croix; Carlos I Batthyany; Bruce A Freeman; Francisco J Schopfer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-09
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-03-25     Revised Date:  2014-05-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44071-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Fatty Acids / metabolism*
Humans
Linoleic Acid / metabolism*
Mice
Mice, Inbred C57BL
Mitochondria / metabolism
Nitrates / metabolism*
Nitric Oxide / metabolism
Nitrites / metabolism*
Signal Transduction
Grant Support
ID/Acronym/Agency:
K99 HL095769/HL/NHLBI NIH HHS; P01-HL103455/HL/NHLBI NIH HHS; P30-DK072506/DK/NIDDK NIH HHS; R00 HL095769/HL/NHLBI NIH HHS; R01 AT006822/AT/NCCAM NIH HHS; R01 AT006822-01/AT/NCCAM NIH HHS; R01 HL064937/HL/NHLBI NIH HHS; R01-HL058115/HL/NHLBI NIH HHS; R01-HL64937/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Nitrates; 0/Nitrites; 31C4KY9ESH/Nitric Oxide; 9KJL21T0QJ/Linoleic Acid
Comments/Corrections

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