Document Detail


Conidiation rhythm and light entrainment in superoxide dismutase mutant in Neurospora crassa.
MedLine Citation:
PMID:  18084778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Conidial formation in the filamentous fungus Neurospora crassa is regulated by nutritional conditions, light, and the circadian clock. We found that a sod-1 mutant, with a defective superoxide dismutase catalyzing the conversion of superoxide to hydrogen peroxide, had a slightly shorter period length than the wild type and clear conidial banding similar to a mutant of band (bd). However, unlike the bd mutant, the sod-1 mutant could sustain conidial banding with light pulses on a nutrient-rich medium, which involved an enhancement of the light-induced transcription of frequency (frq). sod-1 was hypersensitive to entrainment of the conidiation rhythm by light in race tubes. Furthermore, a frq(10); sod-1 double mutant showed conidiation rhythm in darkness and could be synchronized to light/dark cycles by the masking effect of light. These genetic analyses suggested that intracellular reactive oxygen species (ROS) act on circadian conidiation via multiple circadian clocks and output pathways.
Authors:
Yusuke Yoshida; Takashi Maeda; Bumkyu Lee; Kohji Hasunuma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-13
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  279     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-11     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  193-202     Citation Subset:  IM    
Affiliation:
Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Circadian Rhythm*
Fungal Proteins / genetics
Gene Expression Regulation, Fungal / radiation effects
Mutation*
Neurospora crassa / genetics,  physiology,  radiation effects*
RNA, Messenger / genetics,  metabolism
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Spores, Fungal / genetics,  growth & development,  radiation effects*
Superoxide Dismutase / genetics*
Chemical
Reg. No./Substance:
0/FRQ protein, Neurospora crassa; 0/Fungal Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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