| Congruence of total and perfused capillary network in rat brains. | |
| | |
MedLine Citation:
|
PMID: 1688741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In awake normocapnic rats, the density of the total and of the perfused capillary network was determined in 10 brain areas. The density of perfused capillaries was measured by using fluorescein isothiocyanate (FITC) globulin or Evans blue as intravenous marker and by fluorescent microscopy. The density of morphologically existing capillaries was determined according to either the histochemical alkaline phosphatase method or a newly developed immunohistochemical fluorescent method that allows marking of the capillary wall constituent fibronectin with a primary antibody directed against fibronectin. This antibody is made visible by a second FITC-coupled antibody (indirect immunofluorescence). Comparison of perfused and existing capillary counts revealed high congruence when fluorescent results were compared. In contrast, the alkaline phosphatase technique yielded capillary counts that were consistently 30% lower than the fibronectin and the FITC globulin counts. The identity of the perfused and the morphologically existing capillary network could be confirmed by a newly developed double-staining technique. First, the perfused capillaries were quantified by intravascular Evans blue. Then, the existing capillaries were relocated in the same measuring field by the fibronectin technique. Such double staining resulted in identical capillary counts in 97% of all cases. The following conclusions have been reached: 1) Fluorescent methods show a perfusion of virtually all capillaries in the brain of the awake normocapnic rat. 2) The alkaline phosphatase technique appears to underestimate the capillary density in the rat brain. |
| | |
Authors:
|
U Göbel; H Theilen; W Kuschinsky |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Circulation research Volume: 66 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1990 Feb |
Date Detail:
|
Created Date: 1990-03-12 Completed Date: 1990-03-12 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 271-81 Citation Subset: IM |
Affiliation:
|
Department of Physiology, University of Bonn, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acid-Base Equilibrium Alkaline Phosphatase / metabolism Animals Antibodies / immunology Capillaries Cerebrovascular Circulation* Evans Blue / diagnostic use Fibronectins / immunology Fluorescein-5-isothiocyanate Fluoresceins / diagnostic use Fluorescent Antibody Technique Hemodynamics Male Perfusion Photography Rats Rats, Inbred Strains Thiocyanates / diagnostic use gamma-Globulins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies; 0/Fibronectins; 0/Fluoresceins; 0/Thiocyanates; 0/gamma-Globulins; 314-13-6/Evans Blue; 3326-32-7/Fluorescein-5-isothiocyanate; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The CR2 receptor (CD21) shows increased expression in the more differentiated cells of an antigen-sp...
Next Document: Low density lipoprotein transport across a microvascular endothelial barrier after permeability is i...