| Electron microscopy analysis of histone acetylation and DNA strand breaks in mouse elongating spermatids using a dual labelling approach. | |
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MedLine Citation:
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PMID: 20860633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromatin remodelling steps in mammalian spermatids include posttranslational modifications of histones and DNA fragmentation. Histone H4 hyperacetylation (AcH4) establishes a chromatin state that facilitates DNA repair in somatic cells. So we sought to determine whether a similar link exists in spermatids by combining immunogold labelling with detection of DNA strand breaks, making use of gold particles of different sizes. DNA strand breaks were not detected in the vicinity of AcH4 chromatin, suggesting that this modified histone may not be involved in the aetiology of DNA fragmentation and repair in spermatids. The AcH4 reactivity, however, indicates that chromatin remodelling is distributed throughout the nucleus. |
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Authors:
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G Bikond Nkoma; F Leduc; L Jaouad; Guylain Boissonneault |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Andrologia Volume: 42 ISSN: 1439-0272 ISO Abbreviation: Andrologia Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2011-01-10 Revised Date: 2011-12-20 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: Germany |
Other Details:
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Languages: eng Pagination: 322-5 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Animals Chromatin / metabolism, ultrastructure Chromatin Assembly and Disassembly DNA Breaks* DNA Repair Histones / chemistry, metabolism* Immunohistochemistry / methods* Male Mice Microscopy, Electron / methods* Spermatids / metabolism*, ultrastructure* Testis / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Histones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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