Document Detail


Congenic method in the chick limb buds by electroporation.
MedLine Citation:
PMID:  18638168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble-shooting techniques are described.
Authors:
Takayuki Suzuki; Toshihiko Ogura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  50     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-21     Completed Date:  2008-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  459-65     Citation Subset:  IM    
Affiliation:
Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. taka@idac.tohoku.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning
Chick Embryo
DNA / chemistry
Developmental Biology / methods*
Electroporation / methods*
Gene Expression Regulation, Developmental
Genetic Techniques
Genetic Vectors
Green Fluorescent Proteins / metabolism
Limb Buds / metabolism*
Models, Anatomic
Models, Genetic
Tungsten / chemistry
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; 7440-33-7/Tungsten; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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