Document Detail


Congenic mapping and sequence analysis of the Renin locus.
MedLine Citation:
PMID:  23460292     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Renin was the first blood pressure (BP) quantitative trait locus mapped by linkage analysis in the rat. Subsequent BP linkage and congenic studies capturing different portions of the renin region have returned conflicting results, suggesting that multiple interdependent BP loci may be residing in the chromosome 13 BP quantitative trait locus that includes Renin. We used SS-13(BN) congenic strains to map 2 BP loci in the Renin region (chr13: 45.2-49.0 Mb). We identified a 1.1-Mb protective Brown Norway region around Renin (chr13: 46.1-47.2 Mb) that significantly decreased BP by 32 mm Hg. The Renin protective BP locus was offset by an adjacent hypertensive locus (chr13: 47.2-49.0 Mb) that significantly increased BP by 29 mm Hg. Sequence analysis of the protective and hypertensive BP loci revealed 1433 and 2063 variants between Dahl salt-sensitive/Mcwi and Brown Norway rats, respectively. To further reduce the list of candidate variants, we regenotyped an overlapping SS-13(SR) congenic strain (S/renrr) with a previously reported BP phenotype. Sequence comparison among Dahl salt-sensitive, Dahl R, and Brown Norway reduced the number of candidate variants in the 2 BP loci by 42% for further study. Combined with previous studies, these data suggest that at least 4 BP loci reside within the 30-cM chromosome 13 BP quantitative trait locus that includes Renin.
Authors:
Michael J Flister; Matthew J Hoffman; Prajwal Reddy; Howard J Jacob; Carol Moreno
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2013-03-04
Journal Detail:
Title:  Hypertension     Volume:  61     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-14     Completed Date:  2013-05-07     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  850-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Animals, Congenic
Blood Pressure / physiology*
Chromosome Mapping
Disease Models, Animal
Hypertension / genetics*,  metabolism,  physiopathology
Quantitative Trait Loci
Rats
Rats, Inbred BN
Rats, Inbred Dahl
Renin / genetics*,  metabolism
Sequence Analysis
Grant Support
ID/Acronym/Agency:
5P01HL082798-07/HL/NHLBI NIH HHS; 5T32HL007792/HL/NHLBI NIH HHS; P01 HL082798/HL/NHLBI NIH HHS; T32 HL007792/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.23.15/Renin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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