Document Detail

Conformational studies on histone H3 and its CNBr peptides.
MedLine Citation:
PMID:  891546     Owner:  NLM     Status:  MEDLINE    
Histone H3 and H3 peptides 1--120, 1-90, 91-135, 91-120 and 121-135 have been prepared and examined for salt-induced conformational changes by circular dichroism measurements. It was found that reduced histone H3 and the reduced peptides 1-120, 91-135 and 91-120 exhibit biphasic changes with the formation of alpha-helix and beta structures. H3 peptide 1-90, on increasing the ionic strength to moderately high levels, monophasically formed appreciable quantities of alpha-helix and beta structures, while peptide 121-135 remained unfolded under all ionic strengths examined. All the above peptides except 121-135 also aggregate when the ionic strength is raised. The salt-induced near-ultraviolet circular dichroic spectra of histone H3 and peptide 1-90 were found to be very similar, suggesting that the conformational changes induced in the peptide 1-90 are essentially the same as those observed for the intact histone. These results support the contention that the polypeptide segments of this histone interact initially by parallel self-association followed by the formation of even larger aggregates on a longer scale.
G Morris; P N Lewis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  77     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  471-7     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Circular Dichroism
Cyanogen Bromide
Macromolecular Substances
Peptide Fragments
Protein Conformation
Spectrophotometry, Ultraviolet
Thymus Gland
Reg. No./Substance:
0/Amino Acids; 0/Histones; 0/Macromolecular Substances; 0/Peptide Fragments; 506-68-3/Cyanogen Bromide

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