Document Detail


Conformational states of the kinase Lck regulate clustering in early T cell signaling.
MedLine Citation:
PMID:  23202272     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Phosphorylation of the T cell antigen receptor (TCR) by the tyrosine kinase Lck is an essential step in the activation of T cells. Because Lck is constitutively active, spatial organization may regulate TCR signaling. Here we found that Lck distributions on the molecular level were controlled by the conformational states of Lck, with the open, active conformation inducing clustering and the closed, inactive conformation preventing clustering. In contrast, association with lipid domains and protein networks were not sufficient or necessary for Lck clustering. Conformation-driven Lck clustering was highly dynamic, so that TCR triggering resulted in Lck clusters that contained phosphorylated TCRs but excluded the phosphatase CD45. Our data suggest that Lck conformational states represent an intrinsic mechanism for the intermolecular organization of early T cell signaling.
Authors:
Jérémie Rossy; Dylan M Owen; David J Williamson; Zhengmin Yang; Katharina Gaus
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-02
Journal Detail:
Title:  Nature immunology     Volume:  -     ISSN:  1529-2916     ISO Abbreviation:  Nat. Immunol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100941354     Medline TA:  Nat Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Centre for Vascular Research and Australian Centre for Nanomedicine, University of New South Wales, Sydney, Australia.
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