| Conformational order of specific phospholipids in human erythrocytes: correlations with changes in cell shape. | |
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MedLine Citation:
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PMID: 9012681 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acyl chain perdeuterated dimyristoylphosphatidylcholine (DMPC-d54) and dimyristoyphosphatidylserine (DMPS-d54) were incorporated into human erythrocytes. Light microscopy demonstrated that erythrocytes incubated with an equimolar mixture of DMPC-d54/DMPS or DMPC/DMPS-d54 remained mostly discocytic whereas cells incubated with either DMPC-d54 or DMPS-d54 alone became echinocytic or stomatocytic, respectively. Cells in which the aminophospholipid translocating protein was inhibited became echinocytic when incubated with DMPS-d54. Fourier transform infrared (FTIR) spectroscopy was used to monitor conformational order in the acyl chains of the incorporated phospholipid, as detected through the asymmetric CD2 stretching vibrations in the intact cells. In cells incubated with equimolar mixtures of DMPC-d54/DMPS or DMPC/DMPS-d54, the deuterated species exhibited no thermotropic phase transitions but revealed chain order intermediate between the gel and liquid-crystal states. In contrast, DMPS-d54 incorporated into the outer leaflet of echinocytic erythrocytes was conformationally ordered while the same species incorporated into the inner leaflet of stomatocytic erythrocytes was highly disordered at all temperatures studied. Finally, DMPC-d54 incorporated into the outer leaflet of echinocytic erythrocytes exhibited a phase transition, suggesting that this species persists in domains. These data indicate that the acyl chain conformational order of specific phospholipids in the intact human erythrocyte is changed with alterations in cell morphology. |
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Authors:
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D J Moore; R H Sills; R Mendelsohn |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 36 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-02-26 Completed Date: 1997-02-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 660-4 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Newark College of Arts and Sciences, Rutgers University, New Jersey 07102, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dimyristoylphosphatidylcholine Erythrocyte Deformability* Erythrocytes / chemistry, cytology* Humans Models, Molecular Phosphatidylserines / blood Phospholipids / blood* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylserines; 0/Phospholipids; 13699-48-4/Dimyristoylphosphatidylcholine; 64023-32-1/dimyristoylphosphatidylserine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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