| Conformational analysis of heparin epoxide in aqueous solution. An NMR relaxation study. | |
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MedLine Citation:
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PMID: 8548784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1H and 13C NMR relaxation measurements at various magnetic fields have been used to characterize the nature of overall and internal motions in heparin epoxide in aqueous solution. A two-dimensional homonuclear NOESY experiment showed a considerable number of cross-relaxing protons in the molecule. The inter-proton distances calculated from NOE data were compared with those obtained by molecular mechanics calculations. Several discrepancies between the experimental and the theoretical inter-proton distances as well as the variations in 13C spin-lattice relaxation times, measured at two magnetic fields, indicated that the polysaccharide tumbles anisotropically in solution. The rates of overall and internal motions as well as the order parameters have been calculated using a model-free spectral density function. The numerical values indicate that the correlation times which characterize overall molecular motion are outside the extreme narrowing limit (tau parallel = 8 x 10(-10) s and tau perpendicular = 4.2 x 10(-8) s) and that internal motion correlation time is on a picosecond timescale. |
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Authors:
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M Hricovíni; M Guerrini; G Torri; S Piani; F Ungarelli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 277 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-02-21 Completed Date: 1996-02-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 11-23 Citation Subset: IM |
Affiliation:
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Istituto Scientifico di Chimica e Biochimica G. Ronzoni, Milan, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Conformation Carbohydrate Sequence Epoxy Compounds / chemistry*, metabolism Heparin / analogs & derivatives*, chemistry*, metabolism Magnetic Resonance Spectroscopy Molecular Sequence Data Molecular Structure |
| Chemical | |
Reg. No./Substance:
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0/Epoxy Compounds; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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