Document Detail

Conformational analysis of heparin epoxide in aqueous solution. An NMR relaxation study.
MedLine Citation:
PMID:  8548784     Owner:  NLM     Status:  MEDLINE    
1H and 13C NMR relaxation measurements at various magnetic fields have been used to characterize the nature of overall and internal motions in heparin epoxide in aqueous solution. A two-dimensional homonuclear NOESY experiment showed a considerable number of cross-relaxing protons in the molecule. The inter-proton distances calculated from NOE data were compared with those obtained by molecular mechanics calculations. Several discrepancies between the experimental and the theoretical inter-proton distances as well as the variations in 13C spin-lattice relaxation times, measured at two magnetic fields, indicated that the polysaccharide tumbles anisotropically in solution. The rates of overall and internal motions as well as the order parameters have been calculated using a model-free spectral density function. The numerical values indicate that the correlation times which characterize overall molecular motion are outside the extreme narrowing limit (tau parallel = 8 x 10(-10) s and tau perpendicular = 4.2 x 10(-8) s) and that internal motion correlation time is on a picosecond timescale.
M Hricovíni; M Guerrini; G Torri; S Piani; F Ungarelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  277     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-02-21     Completed Date:  1996-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  11-23     Citation Subset:  IM    
Istituto Scientifico di Chimica e Biochimica G. Ronzoni, Milan, Italy.
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Epoxy Compounds / chemistry*,  metabolism
Heparin / analogs & derivatives*,  chemistry*,  metabolism
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
Reg. No./Substance:
0/Epoxy Compounds; 9005-49-6/Heparin

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