| Conformational analysis of the Campylobacter jejuni porin. | |
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MedLine Citation:
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PMID: 7543469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The major outer membrane protein (MOMP) of Campylobacter jejuni was purified to homogeneity by selective solubilization and fast protein liquid chromatography. The amino acid composition of the MOMP indicates the presence of cysteine residues. The amino-terminal sequence, determined over 31 residues, shows no significant homology with any other porin from gram-negative bacteria except in a discrete region. Immunocross-reactivity between Escherichia coli OmpC and the MOMP was analyzed, and a common antigenic site between these two porins was identified with an anti-peptide antibody. From circular dichroism and immunological investigations, the existence of a stable folded monomer, containing a high level of beta-sheet secondary structure, is evident. Conformational analyses show the presence of a native trimeric state generated by association of the three folded monomers; the stability of this trimer is reduced compared with that of E. coli porins. This study clearly reveals that the C. jejuni MOMP is related to the family of trimeric bacterial porins. |
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Authors:
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J M Bolla; E Loret; M Zalewski; J M Pagés |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 177 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1995 Aug |
Date Detail:
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Created Date: 1995-09-13 Completed Date: 1995-09-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4266-71 Citation Subset: IM |
Affiliation:
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UPR 9027, I. F. R. C1, Centre National de la Recherche Scientifique, Marseille, France. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L24960; X73237 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Antigens, Bacterial / immunology Bacterial Outer Membrane Proteins / immunology Bacterial Proteins* Campylobacter jejuni / chemistry*, immunology Circular Dichroism Cross Reactions / immunology Epitopes / immunology Escherichia coli Molecular Sequence Data Porins / chemistry* Protein Conformation Sequence Alignment Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Epitopes; 0/Porins; 0/major outer membrane protein, Campylobacter jejuni |
| Comments/Corrections | |
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