| Conformation of somatostatin using scalar coupling constants from 270 and 600 MHz simulated proton magnetic resonance spectra. | |
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MedLine Citation:
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PMID: 6132629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The conformation of the 14 amino acid peptide hormone somatostatin in aqueous solution was investigated through a proton magnetic resonance (PMR) scalar coupling analysis. Experiments were performed at two fields, 270 and 600 MHz, and included double and triple resonance difference scalar decoupling, resolution enhancement and computer simulation. The agreement between simulated and observed spectra at both fields provided support for the correctness of the analysis. The resultant scalar coupling constants, 3J alpha H-NH and 3J alpha B, gave information on the backbone (phi) and side chain (chi 1) torsional angles, respectively, which eliminated either of the proposed conformations of somatostatin as describing a predominant conformer of the molecule in solution under our conditions. |
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Authors:
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L A Buffington; V Garsky; J Rivier; W A Gibbons |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 41 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-06-10 Completed Date: 1983-06-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 299-304 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Magnetic Resonance Spectroscopy
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methods Protein Conformation Somatostatin* |
| Grant Support | |
ID/Acronym/Agency:
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1-F32 AM6089/AM/NIADDK NIH HHS; CM 295.95/CM/NCI NIH HHS; RR 00292/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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51110-01-1/Somatostatin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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