Document Detail

Conformation types of ubiquitin [M+8H]8+ Ions from water:methanol solutions: evidence for the N and A States in aqueous solution.
MedLine Citation:
PMID:  22315998     Owner:  NLM     Status:  MEDLINE    
Ion mobility and mass spectrometry measurements are used to examine the gas-phase populations of [M+8H](8+) ubiquitin ions formed upon electrospraying 20 different solutions from 100:0 to 5:95 water:methanol that are maintained at pH = 2.0. Over this range of solution conditions, mobility distributions for the +8 charge state show substantial variations. Here we develop a model that treats the combined measurements as one data set. By varying the relative abundances of a discrete set of conformation types, it is possible to represent distributions obtained from any solution. For solutions that favor the well-known A-state ubiquitin, it is possible to represent the gas-phase distributions with seven conformation types. Aqueous conditions that favor the native structure require four more structural types to represent the distribution. This analysis provides the first direct evidence for trace amounts of the A state under native conditions. The method of analysis presented here should help illuminate how solution populations evolve into new gas-phase structures as solvent is removed. Evidence for trace quantities of previously unknown states under native solution conditions may provide insight about the relationship of dynamics to protein function as well as misfolding and aggregation phenomena.
Huilin Shi; Nicholas A Pierson; Stephen J Valentine; David E Clemmer
Related Documents :
18291738 - Poly(hydroxyethyl methacrylate-co-methacrylated-beta-cyclodextrin) hydrogels: synthesis...
23423908 - A simple method for producing colloidal palladium nanocrystals: alternating voltage-ind...
23862478 - Microemulsion-mediated solvothermal synthesis and photoluminescent properties of europi...
16273568 - Photophysical properties of bodipy-derived hydroxyaryl fluorescent ph probes in solution.
6405148 - Hypertonic extracellular expansion in anuria.
23189918 - Abc triblock terpolymers exhibiting both temperature- and ph-sensitive micellar aggrega...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-02
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  116     ISSN:  1520-5207     ISO Abbreviation:  J Phys Chem B     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-15     Completed Date:  2012-07-06     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3344-52     Citation Subset:  IM    
Copyright Information:
© 2012 American Chemical Society
Department of Chemistry, Indiana University, 800 Kirkwood Ave., Bloomington, Indiana 47405, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Hydrogen-Ion Concentration
Ions / chemistry
Mass Spectrometry
Methanol / chemistry*
Solutions / chemistry
Ubiquitin / chemistry*
Water / chemistry*
Grant Support
Reg. No./Substance:
0/Ions; 0/Solutions; 0/Ubiquitin; 67-56-1/Methanol; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Too close to home? Experiences of Kurdish refugee interpreters working in UK mental health services.
Next Document:  Spinal cord trauma and the molecular point of no return.