Document Detail


Confocal scanning laser microscopy evidence of biofilms in patients with chronic rhinosinusitis.
MedLine Citation:
PMID:  17603329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The recent detection of bacterial biofilms on the sinus mucosa of patients with chronic rhinosinusitis (CRS) has implicated biofilms in the pathogenesis of this condition. Electron microscopy has been the main modality used to document the presence of biofilms on sinus tissue, however, it has inherent problems associated with tissue preparation and sampling. Recently, Confocal Scanning Laser Micrsocopy (CSLM) has emerged as a noninvasive, nondestructive technique for the analysis of biofilms. This study used CSLM as the means of investigating biofilm presence in CRS patients. STUDY DESIGN AND METHODS: A prospective study comparing the presence of bacterial biofilms on the sinus mucosa of CRS and control patients was conducted using CSLM. Thirty eight CRS patients undergoing endoscopic sinus surgery and nine control patients were enrolled in this study. Demographic and clinical information was recorded from each patient and intraoperatively, sinus culture specimens and mucosal samples were obtained for microbiologic and microscopic examination. RESULTS: Using previously documented CSLM criteria, bacterial biofilms were found in 17 (44%) of the 38 CRS patients. No biofilm structures were evident in any of the controls. Patients having undergone previous sinus surgery seemed to have a higher incidence of biofilms compared with the incidence for those undergoing their first procedure. The difference however was not statistically significant. No correlation between positive bacterial cultures and biofilm presence was observed. CONCLUSIONS: The CSLM detection of biofilms in CRS patients and their absence in controls further supports the hypothesis that biofilms may play a role in the pathogenesis of CRS. This study's lower reported incidence of biofilms compared with that of previous studies might reflect the increased accuracy of biofilm detection with CSLM.
Authors:
Alkis J Psaltis; Kien R Ha; Achim G Beule; Lor Wai Tan; Peter-John Wormald
Related Documents :
6974939 - Spontaneous reversion to normal sinus rhythm after prolonged atrial fibrillation.
8076119 - Right atrial isolation associated with atrial septal closure in patients with atrial se...
17073989 - Combination of pulse therapy with terbinafine tablets and topical terbinafine cream for...
8945039 - Dual chamber pacing with a single lead system: initial clinical results.
8617899 - Prevalence of atrial fibrillation and association of atrial fibrillation with prior and...
16855499 - When is enophthalmos "significant"?
18972769 - Malnutrition in inflammatory bowel disease patients in northern india: frequency and fa...
18424899 - Cardiothoracic ratio, malnutrition, inflammation, and two-year mortality in non-diabeti...
4532969 - Hyperphagia in intestinal disease.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Laryngoscope     Volume:  117     ISSN:  0023-852X     ISO Abbreviation:  Laryngoscope     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-02     Completed Date:  2007-08-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1302-6     Citation Subset:  IM    
Affiliation:
Department of Surgery-Otorhinolayrngology Head and Neck Surgery, The Queen Elizabeth Hospital, and The University of Adelaide, Adelaide, South Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aged
Bacterial Infections / microbiology*
Biofilms*
Chronic Disease
Endoscopy
Female
Humans
Male
Microscopy, Confocal* / methods
Middle Aged
Nasal Mucosa / microbiology*,  pathology*
Prospective Studies
Rhinitis / diagnosis,  pathology*,  surgery
Sinusitis / diagnosis,  pathology*,  surgery

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Structural variations in a single hyaluronan derivative significantly alter wound-healing effects in...
Next Document:  Recovery of vestibular imbalances after vestibular neuritis.