Document Detail

Confocal microscopic localization of constitutive and heat shock-induced proteins HSP70 and HSP27 in the rat heart.
MedLine Citation:
PMID:  11015351     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Heat-shock treatment of rats elevates expression of heat-shock proteins, which play a role in improving the contractile recovery and reducing infarct size in hearts after ischemic injury. However, the location of these proteins in the heart is unknown. METHODS AND RESULTS: Anesthetized rats were heat-shocked by elevation of body temperature to 42 degrees C to 42.5 degrees C for 15 minutes, followed by 24 hours of recovery. Control and heat-shocked hearts were extirpated and perfused briefly with saline followed by 2% paraformaldehyde in PBS. Confocal immunofluorescence microscopy of control hearts revealed that HSP27 was localized in cardiomyocytes in a pattern reminiscent of Z bands and was colocalized with neuronal markers in somata and axons. No obvious change in HSP27 content or distribution occurred after heat shock. Confocal microscopy revealed little or no HSP70 in control hearts. After heat shock, HSP70 was detected neither in cardiomyocytes nor in neuronal elements within the heart, but HSP70 was abundant in small blood vessels found between the ventricular cardiomyocytes. CONCLUSIONS: Heat shock induces a cell type-specific expression of HSP70 in blood vessels but not myocytes or intrinsic cardiac neurons, suggesting that blood vessels play a primary role in myocardial protection.
J P Leger; F M Smith; R W Currie
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation     Volume:  102     ISSN:  1524-4539     ISO Abbreviation:  Circulation     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-06     Completed Date:  2000-10-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1703-9     Citation Subset:  IM    
Department of Anatomy and Neurobiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
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MeSH Terms
Blood Vessels / metabolism
Body Temperature
HSP27 Heat-Shock Proteins
HSP70 Heat-Shock Proteins / metabolism*
Heart / innervation,  physiology
Heat-Shock Proteins*
Microscopy, Confocal
Myocardium / metabolism*
Neoplasm Proteins / metabolism*
Neurons / metabolism*
Rats, Sprague-Dawley
Tissue Distribution
Reg. No./Substance:
0/HSP27 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, rat; 0/Neoplasm Proteins
Comment In:
Circulation. 2001 Nov 27;104(22):E123   [PMID:  11723036 ]

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