Document Detail


Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa.
MedLine Citation:
PMID:  20386810     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.
Authors:
Konrad Meister; Diedrich A Schmidt; Erik Bründermann; Martina Havenith
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-13
Journal Detail:
Title:  The Analyst     Volume:  135     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-25     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1370-4     Citation Subset:  IM    
Affiliation:
Ruhr-Universität Bochum, Physical Chemistry II, Bldg. NC 7/72, Universitätsstr. 150, 44780, Bochum, Germany.
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MeSH Terms
Descriptor/Qualifier:
Humans
Male
Microscopy, Confocal / methods*
Mitochondria / chemistry,  physiology*
Spectrum Analysis, Raman / methods*
Spermatozoa / chemistry,  physiology*
Ultraviolet Rays

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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