| Confirmation and determination of carboxylic acids in root exudates using LC-ESI-MS. | |
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MedLine Citation:
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PMID: 17763514 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Reversed-phase liquid chromatography with UV detection is of limited applicability in the separation and identification of carboxylic acids because of the column's poor separation efficiency and the non-selective nature of the UV detector. To address this issue, RP-LC with electrospray ionization mass spectrometry has been explored for the confirmation and determination of carboxylic acids in plant root exudates, with ESI-MS providing structural information, high selectivity, and high sensitivity. The separation of 10 carboxylic acids (pyruvic, lactic, malonic, maleic, fumaric, succinic, malic, tartaric, trans-aconitic, and citric acid) was performed on a C(18) column using an eluent containing 0.1% (v/v) acetic acid within 10 min, where the acidic eluent not only suppressed the ionization of the carboxylic acids to be retained on the column, but was also compatible with ESI-MS detection. In addition, an additional standard was used to overcome the matrix effect. The results showed that peak areas correlated linearly with the concentration of carboxylic acids over the range 0.05-10 mg/L. The detection limits of target acids (signal-to-noise S/N ratio of 3) ranged from 20 to 30 microg/L. Finally, the proposed method was used for the confirmation and determination of low-molecular-weight carboxylic acids in plant root exudates, and provided a simple analytical procedure, including sample processing, fast separation, and high specificity and sensitivity. |
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Authors:
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Zuliang Chen; Xiaoyin Jin; Qinqping Wang; Yuman Lin; Li Gan; Caixing Tang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of separation science Volume: 30 ISSN: 1615-9306 ISO Abbreviation: J Sep Sci Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-08 Completed Date: 2008-01-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 2440-6 Citation Subset: - |
Affiliation:
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School of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China. ZLchen@fjnu.edu.cn |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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