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Confirmation and determination of carboxylic acids in root exudates using LC-ESI-MS.
MedLine Citation:
PMID:  17763514     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Reversed-phase liquid chromatography with UV detection is of limited applicability in the separation and identification of carboxylic acids because of the column's poor separation efficiency and the non-selective nature of the UV detector. To address this issue, RP-LC with electrospray ionization mass spectrometry has been explored for the confirmation and determination of carboxylic acids in plant root exudates, with ESI-MS providing structural information, high selectivity, and high sensitivity. The separation of 10 carboxylic acids (pyruvic, lactic, malonic, maleic, fumaric, succinic, malic, tartaric, trans-aconitic, and citric acid) was performed on a C(18) column using an eluent containing 0.1% (v/v) acetic acid within 10 min, where the acidic eluent not only suppressed the ionization of the carboxylic acids to be retained on the column, but was also compatible with ESI-MS detection. In addition, an additional standard was used to overcome the matrix effect. The results showed that peak areas correlated linearly with the concentration of carboxylic acids over the range 0.05-10 mg/L. The detection limits of target acids (signal-to-noise S/N ratio of 3) ranged from 20 to 30 microg/L. Finally, the proposed method was used for the confirmation and determination of low-molecular-weight carboxylic acids in plant root exudates, and provided a simple analytical procedure, including sample processing, fast separation, and high specificity and sensitivity.
Authors:
Zuliang Chen; Xiaoyin Jin; Qinqping Wang; Yuman Lin; Li Gan; Caixing Tang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  30     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-08     Completed Date:  2008-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2440-6     Citation Subset:  -    
Affiliation:
School of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China. ZLchen@fjnu.edu.cn
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