Document Detail

Conditionally immortalized oligodendrocyte cell lines migrate to different brain regions and elaborate 'myelin-like' membranes after transplantation into neonatal shiverer mouse brains.
MedLine Citation:
PMID:  8549427     Owner:  NLM     Status:  MEDLINE    
Five immortalized oligodendrocyte cell lines, representing a spectrum of different stages of oligodendrocyte maturation, were transplanted into neonatal shiverer mouse brains and examined for their ability to survive, multiply, and migrate in vivo. Each of the cell lines migrated to different regions of the brain with remarkable consistency when injected into the mouse forebrain, suggesting that the cells might be responding to different environmental cues present in the neonatal mouse brain. These results are consistent with the fact that cells at different stages in the oligodendrocyte lineage probably possess different sets of surface molecules and receptors. Significant differences were also observed in the survival and proliferation of the lines examined, even when the lines tested had quite similar in vitro phenotypes. Interestingly, the cell line with the most mature in vitro phenotype, N20.1, appeared to elaborate membranous processes when transplanted into the brain, reminiscent of oligodendrocytes ensheathing axonal segments. The experiments suggest that these immortalized cells could be useful models to study the cellular and molecular mechanisms involved in the development, maturation and possibly formation of myelin by oligodendrocytes in the mammalian brain.
L M Foster; C Landry; T Phan; A T Campagnoni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental neuroscience     Volume:  17     ISSN:  0378-5866     ISO Abbreviation:  Dev. Neurosci.     Publication Date:  1995  
Date Detail:
Created Date:  1996-02-22     Completed Date:  1996-02-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7809375     Medline TA:  Dev Neurosci     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  160-70     Citation Subset:  IM    
Mental Retardation Research Center, UCLA School of Medicine 90024, USA.
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MeSH Terms
Animals, Newborn
Brain / cytology*,  ultrastructure
Brain Chemistry
Brain Tissue Transplantation / physiology*
Cell Line
Cell Movement / physiology
Cell Transplantation / physiology*
Membranes / metabolism,  ultrastructure
Mice, Neurologic Mutants
Microscopy, Electron
Myelin Sheath / metabolism*,  ultrastructure
Oligodendroglia / metabolism*,  ultrastructure
beta-Galactosidase / metabolism
Grant Support
Reg. No./Substance:

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