Document Detail


Conditional knockdown of hMRS2 results in loss of mitochondrial Mg(2+) uptake and cell death.
MedLine Citation:
PMID:  18384665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) transport, we have established stable cell lines with conditionally expressing shRNAs directed against hMRS2L. The cells expressing shRNA for several generations exhibited lower steady-state levels of free mitochondrial Mg(2+) ([Mg(2+)](m)) and reduced capacity of mitochondrial Mg(2+) uptake than control cells. Long-term expression of shRNAs resulted in loss of mitochondrial respiratory complex I, decreased mitochondrial membrane potential and cell death. We conclude that hMrs2 is the major transport protein for Mg (+) uptake into mitochondria and that expression of hMrs2 is essential for the maintenance of respiratory complex I and cell viability.
Authors:
Martin Piskacek; Ludmila Zotova; Gábor Zsurka; Rudolf J Schweyen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-31
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  13     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-14     Completed Date:  2009-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  693-700     Citation Subset:  IM    
Affiliation:
Department of Genetics, Vienna University, Austria.
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MeSH Terms
Descriptor/Qualifier:
Cation Transport Proteins / metabolism*
Cell Death
Cell Line
Down-Regulation
Gene Knockdown Techniques*
Humans
Magnesium / metabolism*
Membrane Potential, Mitochondrial
Mitochondria / metabolism*
Mitochondrial Membranes / metabolism
Mitochondrial Proteins / metabolism*
RNA, Small Interfering / metabolism
Transfection
Chemical
Reg. No./Substance:
0/Cation Transport Proteins; 0/MRS2 protein, human; 0/Mitochondrial Proteins; 0/RNA, Small Interfering; 7439-95-4/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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