Document Detail


Condensin loaded onto the replication fork barrier site in the rRNA gene repeats during S phase in a FOB1-dependent fashion to prevent contraction of a long repetitive array in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16507999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An average of 200 copies of the rRNA gene (rDNA) is clustered in a long tandem array in Saccharomyces cerevisiae. FOB1 is known to be required for expansion/contraction of the repeats by stimulating recombination, thereby contributing to the maintenance of the average copy number. In Deltafob1 cells, the repeats are still maintained without any fluctuation in the copy number, suggesting that another, unknown system acts to prevent repeat contraction. Here, we show that condensin acts together with FOB1 in a functionally complemented fashion to maintain the long tandem repeats. Six condensin mutants possessing severely contracted rDNA repeats were isolated in Deltafob1 cells but not in FOB1+ cells. We also found that the condensin complex associated with the nontranscribed spacer region of rDNA with a major peak coincided with the replication fork barrier (RFB) site in a FOB1-dependent fashion. Surprisingly, condensin association with the RFB site was established during S phase and was maintained until anaphase. These results indicate that FOB1 plays a novel role in preventing repeat contraction by regulating condensin association and suggest a link between replication termination and chromosome condensation and segregation.
Authors:
Katsuki Johzuka; Masahiro Terasawa; Hideyuki Ogawa; Tomoko Ogawa; Takashi Horiuchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  26     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-01     Completed Date:  2006-06-20     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2226-36     Citation Subset:  IM    
Affiliation:
Laboratory of Genome Dynamics, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki 444-8585, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics*,  metabolism
Carrier Proteins / genetics,  metabolism
Cell Proliferation
Chromosome Segregation
DNA Replication*
DNA, Ribosomal / genetics,  metabolism
DNA, Ribosomal Spacer
DNA-Binding Proteins / genetics*,  metabolism*
Genes, Lethal
Multiprotein Complexes / genetics*,  metabolism
Mutation
Nuclear Proteins / genetics,  metabolism
Protein Subunits
RNA, Ribosomal / genetics*,  metabolism
Repetitive Sequences, Nucleic Acid
S Phase / genetics
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/DNA, Ribosomal; 0/DNA, Ribosomal Spacer; 0/DNA-Binding Proteins; 0/FOB1 protein, S cerevisiae; 0/Multiprotein Complexes; 0/Nuclear Proteins; 0/Protein Subunits; 0/RNA, Ribosomal; 0/SMC2 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/condensin complexes; EC 3.6.1.-/Adenosine Triphosphatases
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