Document Detail


Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.
MedLine Citation:
PMID:  20805030     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.
Authors:
Jia Liu; Carlos A Castañeda; Bryan J Wilkins; David Fushman; T Ashton Cropp
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-08-13
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  20     ISSN:  1464-3405     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2011-02-24     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  5613-6     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Amino Acyl-tRNA Synthetases / metabolism
Benzophenones / metabolism
Escherichia coli / metabolism*
Phenylalanine / analogs & derivatives,  metabolism
Recombinant Proteins / biosynthesis*,  chemistry
Ubiquitin / biosynthesis
Grant Support
ID/Acronym/Agency:
GM065334/GM/NIGMS NIH HHS; GM084396/GM/NIGMS NIH HHS; R01 GM065334/GM/NIGMS NIH HHS; R01 GM084396/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/4-benzoylphenylalanine; 0/Amino Acids; 0/Benzophenones; 0/Recombinant Proteins; 0/Ubiquitin; 47E5O17Y3R/Phenylalanine; EC 6.1.1.-/Amino Acyl-tRNA Synthetases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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