| Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids. | |
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MedLine Citation:
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PMID: 20805030 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures. |
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Authors:
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Jia Liu; Carlos A Castañeda; Bryan J Wilkins; David Fushman; T Ashton Cropp |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-08-13 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: 20 ISSN: 1464-3405 ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2011-02-24 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: England |
Other Details:
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Languages: eng Pagination: 5613-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Amino Acyl-tRNA Synthetases / metabolism Benzophenones / metabolism Escherichia coli / metabolism* Phenylalanine / analogs & derivatives, metabolism Recombinant Proteins / biosynthesis*, chemistry Ubiquitin / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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GM065334/GM/NIGMS NIH HHS; GM084396/GM/NIGMS NIH HHS; R01 GM065334-09/GM/NIGMS NIH HHS; R01 GM084396-01A1/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/4-benzoylphenylalanine; 0/Amino Acids; 0/Benzophenones; 0/Recombinant Proteins; 0/Ubiquitin; 63-91-2/Phenylalanine; EC 6.1.1.-/Amino Acyl-tRNA Synthetases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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