Document Detail

Concurrent specific immunological detection of both primate and rodent forms of the guanine nucleotide binding protein G11 alpha following their coexpression.
MedLine Citation:
PMID:  8038205     Owner:  NLM     Status:  MEDLINE    
The phosphoinositidase C-linked G proteins Gq alpha and G11 alpha are highly similar and comigrate in 10% (w/v) acrylamide SDS-PAGE. Antisera generated against regions common between these G proteins thus detect a composite of the two polypeptides following resolution in such gels. Using SDS-PAGE conditions which allow resolution of Gq alpha and G11 alpha in rodent brain and neuroblastoma cell lines it was observed that primate frontal cortex and neuroblastoma cell lines did not express a polypeptide which comigrated with rodent G11 alpha. Species diversity in G-protein sequences is extremely limited; however, immunoblotting primate cells and frontal cortex with a G11 alpha-specific antiserum demonstrated this to be due to a difference in mobility of rodent and primate G11 alpha under these conditions rather than lack of expression of G11 alpha by primates. A cDNA encoding mouse G11 alpha was transiently expressed in monkey COS-1 cells and membranes from these cells were immunoblotted with antisera able to identify primate and rodent G11 alpha equally, following SDS-PAGE under the resolving conditions. Both mouse and monkey G11 alpha could be detected concurrently and unambiguously following transfection. This is the first demonstration that species variants of the same G protein expressed in a single cell can be detected simultaneously.
G D Kim; G Milligan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1222     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-23     Completed Date:  1994-08-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  369-74     Citation Subset:  IM    
Department of Biochemistry, University of Glasgow, UK.
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MeSH Terms
Cell Line
Cell Membrane / metabolism
Cerebral Cortex / metabolism
GTP-Binding Proteins / analysis*,  isolation & purification,  metabolism
Gene Expression
Guinea Pigs
Tumor Cells, Cultured
Reg. No./Substance:
EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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