| Concurrent detection of minimal residual disease (MRD) in childhood acute lymphoblastic leukaemia by flow cytometry and real-time PCR. | |
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MedLine Citation:
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PMID: 15755280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Minimal (i.e. submicroscopic) residual disease (MRD) predicts outcome in childhood acute lymphoblastic leukaemia (ALL). To be used clinically, MRD assays must be reliable and accurate. Two well-established techniques, flow cytometry (FC) and polymerase chain reaction (PCR), can detect leukaemic cells with a sensitivity of 0.01% (10(-4)). We analysed diagnostic samples of 45 ALL-patients (37 B-lineage ALL, eight T-lineage ALL) by four-colour FC and real-time PCR. Leukaemia-associated immunophenotypes, at a sensitivity of MRD detection by FC at the 0.01% level, were identified in 41 cases (91%); antigen-receptor gene rearrangements suitable for MRD detection with a sensitivity of 0.01% or better by PCR were identified in 38 cases (84%). The combined use of FC and PCR allowed MRD monitoring in all 45 patients. In 105 follow-up samples, MRD estimates by both methods were highly concordant, with a deviation factor of <5 by Bland-Altman analysis. Importantly, the concordance between FC and PCR was also observed in regenerating bone marrow samples containing high proportions of CD19(+) cells, and in samples studied 24 h after collection. We conclude that both MRD assays yield generally concordant results. Their combined use should enable MRD monitoring in virtually all patients and prevent false-negative results due to clonal evolution or phenotypic shifts. |
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Authors:
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Gunter Kerst; Hermann Kreyenberg; Carmen Roth; Catrin Well; Klaus Dietz; Elaine Coustan-Smith; Dario Campana; Ewa Koscielniak; Charlotte Niemeyer; Paul G Schlegel; Ingo Müller; Dietrich Niethammer; Peter Bader |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of haematology Volume: 128 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-09 Completed Date: 2005-04-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: England |
Other Details:
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Languages: eng Pagination: 774-82 Citation Subset: IM |
Affiliation:
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Department of Pediatric Haematology and Oncology, University Children's Hospital, D-72076 Tübingen, Germany. gunter.kerst@med.uni-tuebingen.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Child Child, Preschool Flow Cytometry / methods*, standards Gene Rearrangement, T-Lymphocyte Humans Infant Neoplasm, Residual Phenotype Polymerase Chain Reaction / methods*, standards Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology* Sensitivity and Specificity Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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