| Conclusive evidence that very-long-chain fatty acids are oxidized exclusively in peroxisomes in human skin fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 1872864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have investigated the contribution of peroxisomes and mitochondria to the beta-oxidation of palmitate (C16:0) and cerotate (C26:0) in intact human skin fibroblasts. The oxidation of both fatty acids was found to be inhibited by rotenone plus antimycin and cyanide, respectively, although to a different extent. When 2-[5-(4-chlorophenyl)pentyl]-oxirane-2-carboxylate (POCA) was used to specifically block carnitine palmitoyltransferase I, it was found that palmitate beta-oxidation was inhibited almost completely whereas cerotate beta-oxidation was not affected. Since carnitine palmitoyltransferase is essential for the oxidation of fatty acids in mitochondria this result provides conclusive evidence that oxidation of very-long-chain fatty acids is initiated in peroxisomes and not in mitochondria. |
| | |
Authors:
|
B S Jakobs; R J Wanders |
Related Documents
:
|
7988704 - Transient induction of fatty acid synthase in rat liver after removal of a peroxisome p... 9717014 - Efficiency of metabolic screening in childhood cardiomyopathies. 11339654 - Disorders of fatty acid transport and mitochondrial oxidation: challenges and dilemmas ... 2427264 - Accumulation and defective beta-oxidation of very long chain fatty acids in zellweger's... 4018504 - Metabolism of lithocholic and chenodeoxycholic acids in the squirrel monkey. 8157634 - Phospholipids of rhizobium contain node-determined highly unsaturated fatty acid moieties. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 178 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1991 Aug |
Date Detail:
|
Created Date: 1991-09-18 Completed Date: 1991-09-18 Revised Date: 2013-02-11 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 842-7 Citation Subset: IM |
Affiliation:
|
Dept. of Clinical Biochemistry, University Hospital Amsterdam, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antimycin A
/
analogs & derivatives,
pharmacology Cells, Cultured Epoxy Compounds / pharmacology Fatty Acids / metabolism* Fibroblasts / drug effects, metabolism Humans Hypoglycemic Agents / pharmacology Kinetics Microbodies / drug effects, metabolism* Oxidation-Reduction Palmitic Acid Palmitic Acids / metabolism* Potassium Cyanide / pharmacology Rotenone / pharmacology Skin / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Epoxy Compounds; 0/Fatty Acids; 0/Hypoglycemic Agents; 0/Palmitic Acids; 11118-72-2/antimycin; 151-50-8/Potassium Cyanide; 57-10-3/Palmitic Acid; 642-15-9/Antimycin A; 78573-70-3/B 807-27; 83-79-4/Rotenone; D42CQN6P36/hexacosanoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spermidine regulation of ornithine decarboxylase synthesis by a GC-rich sequence of the 5'-untransla...
Next Document: Identification of a soluble enzyme from C3H/10T1/2 cells which is inhibited by the Bowman-Birk prote...