| Concerted activities of nitric oxide synthases and NADPH oxidases in PLB-985 cells. | |
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MedLine Citation:
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PMID: 17655824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative stress is a metabolic situation used by immune cells to provide protection against infection. Under activation by threatening elements, phagocytes produce chemically toxic molecules, namely the reactive oxygen species (ROS) and reactive nitrogen species (RNS). This mechanism involves two types of enzymes: NAPDH oxidases (NOX) and NO synthases (NOS), which activities are versatile and not fully understood yet. In this regard, we studied in real-time the release of bursts of ROS and RNS by single PLB-985 cells, originating from a myeloid cell line prone to differentiate into neutrophil or monocyte-like phagocytes. A selective electrochemical detection of each ROS or RNS was conducted at platinized carbon fiber microelectrodes positioned at micrometric distances from single cells. Our results show (1) the existence of a NO synthase activity in PLB-985 cells and (2) the ability of NO synthases to provide a NOX activity in cells where NADPH oxidase (NOX2) is knocked out. |
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Authors:
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Yann Verchier; Bernard Lardy; Minh Vu Chuong Nguyen; Françoise Morel; Stéphane Arbault; Christian Amatore |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-20 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 361 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-07 Completed Date: 2007-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 493-8 Citation Subset: IM |
Affiliation:
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Laboratoire PASTEUR, Ecole Normale Supérieure, CNRS UMR 8640, Département de Chimie, 24 rue Lhomond, 75005 Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidines
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pharmacology Benzylamines / pharmacology Cell Line Cell Membrane / drug effects, enzymology NADPH Oxidase / metabolism* NG-Nitroarginine Methyl Ester / pharmacology Nitric Oxide Synthase / antagonists & inhibitors, metabolism* Nitric Oxide Synthase Type II / antagonists & inhibitors Oxidative Stress / drug effects Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism Respiratory Burst / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Benzylamines; 0/N-(3-(aminomethyl)benzyl)acetamidine; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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