Document Detail

Conceptual comparison of two computer models of corpuscle sectioning and of two algorithms for correction of ploidy measurements in tissue sections.
MedLine Citation:
PMID:  10696456     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare two computer models of corpuscle sectioning and two algorithms for correction of ploidy measurements in tissue sections.
STUDY DESIGN: Two models of corpuscle sectioning (the computed corpuscle sectioning program [CCSP] [Analyt Quant Cytol Histol 1997;19:376-386] and the ellipsoid sectioning program [ESP]) were run on a personal computer to generate synthetic corpuscle section data that model the sectioned nuclei in a tissue section. These synthetic data were analyzed by two algorithms for correction of ploidy measurements in tissue sections: the reference curve method (RCM) (Analyt Quant Cytol Histol 1997;19:376-386) and the method of McCready and Papadimitriou (MMP) (Analyt Quant Cytol 1983;5:117-123) for a variety of choices of section thickness and of nuclear section profile selection criteria.
RESULTS: Previous recommendations (Analyt Quant Cytol Histol 1999;21:103-112) for optimization of ploidy analysis in tissue sections (selection of only center-containing sections of nuclei in ultrathin sections with a selection bias in favor of elliptical nuclear section profiles) are valid regardless of which corpuscle sectioning model and correction algorithm are employed. Perimeter correction may be desirable or necessary in some cases. The RCM has very significant advantages over the MMP, and the CCSP is more applicable to actual ploidy analysis than is the ESP.
CONCLUSION: The RCM always should be used to correct ploidy measurements in tissue sections. The MMP should not be used as the sole method but, when used, should be used with and interpreted in the context of the RCM.
J A Freed
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  22     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-04-12     Completed Date:  2000-04-12     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-25     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / genetics
Computer Simulation
DNA / blood*
Erythrocytes / ultrastructure*
Evaluation Studies as Topic
Image Cytometry / methods*
Image Processing, Computer-Assisted*
Reg. No./Substance:

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