Document Detail


Concentration and M/G ratio influence the physiochemical and mechanical properties of alginate constructs for tissue engineering.
MedLine Citation:
PMID:  20799207     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The diffusion and mechanical properties of calcium alginate gels were determined using constructs of different alginate concentrations and guluronic acid contents. It was found that the diffusion of small molecules such as sulphate, glucose and thymidine was not impeded by any of the alginates tested at concentrations of 1% and 3% (w/v). By contrast, the diffusion of large molecules, including insulin like growth factor-1 (IGF-1), human growth hormone and bovine serum albumin was impeded by alginate. This effect was enhanced with increasing alginate concentration, but was less evident for alginates with increased guluronic acid content. These findings have significant implications in tissue engineering where cells such as chondrocytes depend on the supply of factors such as IGF-1 to remain viable. An increase in both alginate concentration and guluronic acid content also increased the compressive properties, as determined by both tangent and equilibrium modulus, of alginate constructs. Although the 3% alginate constructs exhibited enhanced stiffness compared to some reported cartilage substitute biomaterials, such as PGA, their absolute values were still appreciably less stiff than articular cartilage.
Authors:
B Enobakhare; D L Bader; D A Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied biomaterials & biomechanics : JABB     Volume:  4     ISSN:  1722-6899     ISO Abbreviation:  J Appl Biomater Biomech     Publication Date:    2006 May-Aug
Date Detail:
Created Date:  2010-08-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101180437     Medline TA:  J Appl Biomater Biomech     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  87-96     Citation Subset:  -    
Affiliation:
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London - UK.
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