Document Detail


Concentration of mammalian genomic DNA using two-phase aqueous micellar systems.
MedLine Citation:
PMID:  19061237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The concentration of biomarkers, such as DNA, prior to a subsequent detection step may facilitate the early detection of cancer, which could significantly increase chances for survival. In this study, the partitioning behavior of mammalian genomic DNA fragments in a two-phase aqueous micellar system was investigated using both experiment and theory. The micellar system was generated using the nonionic surfactant Triton X-114 and phosphate-buffered saline (PBS). Partition coefficients were measured under a variety of conditions and compared with our theoretical predictions. With this comparison, we demonstrated that the partitioning behavior of DNA fragments in this system is primarily driven by repulsive, steric, excluded-volume interactions that operate between the micelles and the DNA fragments, but is limited by the entrainment of micelle-poor, DNA-rich domains in the macroscopic micelle-rich phase. Furthermore, the volume ratio, that is, the volume of the top, micelle-poor phase divided by that of the bottom, micelle-rich phase, was manipulated to concentrate DNA fragments in the top phase. Specifically, by decreasing the volume ratio from 1 to 1/10, we demonstrated proof-of-principle that the concentration of DNA fragments in the top phase could be increased two- to nine-fold in a predictive manner.
Authors:
Foad Mashayekhi; Aaron S Meyer; Stacey A Shiigi; Vu Nguyen; Daniel T Kamei
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  102     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-02-26     Completed Date:  2009-08-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1613-23     Citation Subset:  IM    
Copyright Information:
2008 Wiley Periodicals, Inc.
Affiliation:
Department of Bioengineering, University of California, Los Angeles, 90095, USA.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Chemical Fractionation / methods*
DNA / isolation & purification*
Hela Cells
Humans
Micelles*
Models, Chemical
Neoplasms / diagnosis
Polyethylene Glycols / chemistry
Salts / chemistry
Temperature
Time Factors
Tumor Markers, Biological / isolation & purification
Chemical
Reg. No./Substance:
0/Micelles; 0/Polyethylene Glycols; 0/Salts; 0/Tumor Markers, Biological; 9007-49-2/DNA; 9036-19-5/Nonidet P-40

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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